AKR1C4 蛋白, Human (His)

AKR1C4 Protein, Human (His) is a human recombinant AKR1C4 with a N-Terminal His-tag. AKR1C4 Protein, Human (His) is derived from E. coli and corresponding to the amino acids 1-323 of human Aldo-keto Reductase 1C4/AKR1C4.

醛酮还原酶 1C4 (AKR1C4) 是醛酮还原酶 (AKR) 超家族的成员。AKR1C4 催化各种 3α-羟基类固醇和前列腺素的可逆氧化还原，以及芳香烃和脂环醇的反式二氢二醇的氧化。AKR1C4 充当 NADPH 依赖性 3-、17- 和 20- 酮类固醇还原酶以及 3α-、17β- 和 20α-羟基类固醇氧化酶。AKR1C4 将 11-酮二氢睾酮 (11KDHT) 还原为 3α- 和 3β-羟基代谢物。AKR1C4 对于主要通过睾酮合成和清除的类固醇激素代谢非常重要。AKR1C4 实际上具有肝脏特异性，其高 Kcat/Km 使该酶能够稳定地形成 5α/5β-四氢类固醇^[1][2]。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

P17516 (M1-Y323)

1109  [NCBI]

HHHHHHMDPKYQRVELNDGHFMPVLGFGTYAPPEVPRNRAVEVTKLAIEAGFRHIDSAYLYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWCTFFQPQMVQPALESSLKKLQLDYVDLYLLHFPMALKPGETPLPKDENGKVIFDTVDLSATWEVMEKCKDAGLAKSIGVSNFNCRQLEMILNKPGLKYKPVCNQVECHPYLNQSKLLDFCKSKDIVLVAHSALGTQRHKLWVDPNSPVLLEDPVLCALAKKHKQTPALIALRYQLQRGVVVLAKSYNEQRIRENIQVFEFQLTSEDMKVLDGLNRNYRYVVMDFLMDHPDYPFSDEY

Approximately 38.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filter solution of 20 mM Tris, 150 mM NaCl, pH 8.0 or 20 mM Tris-HCl, 150 mM NaCl, 10% Sucrose, 0.05% Tween 80, pH 9.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Endo S, et al. Human dehydrogenase/reductase SDR family member 11 (DHRS11) and aldo-keto reductase 1C isoforms in comparison: Substrate and reaction specificity in the reduction of 11-keto-C19-steroids. J Steroid Biochem Mol Biol. 2020 May;199:105586.  [Content Brief]

  [2]. Penning TM, et al. Human 3alpha-hydroxysteroid dehydrogenase isoforms (AKR1C1-AKR1C4) of the aldo-keto reductase superfamily: functional plasticity and tissue distribution reveals roles in the inactivation and formation of male and female sex hormones. Biochem J. 2000 Oct 1;351(Pt 1):67-77.  [Content Brief]