ALDH1A3 蛋白, Human (His)

ALDH1A3 Protein, Human (His) is a human recombinant ALDH1A3 with a His tag at the N-terminus. ALDH1A3 Protein, Human (His) is produced in E. coli and the target gene encoding Met1-Pro512 is expressed.

醛脱氢酶 1-A3 (ALDH1A3) 使用 NAD+ 催化视黄醛氧化为多效因子视黄酸。ALDH1A3通过将全反式视黄醛氧化为视黄酸来参与人体细胞的各种生理过程。ALDH1A3 影响癌症干细胞内的多种生物学特性，并可以作为这些细胞的标记。ALDH1A3 在早期胚胎头部和前脑发育过程中被差异激活。ALDH1A3 被认为是乳腺癌的靶标。在高级胶质瘤 (HGG)^[1][2] 中观察到 ALDH1A3 高表达。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

P47895 (M1-P512)

220  [NCBI]

HHHHHHMATANGAVENGQPDRKPPALPRPIRNLEVKFTKIFINNEWHESKSGKKFATCNPSTREQICEVEEGDKPDVDKAVEAAQVAFQRGSPWRRLDALSRGRLLHQLADLVERDRATLAALETMDTGKPFLHAFFIDLEGCIRTLRYFAGWADKIQGKTIPTDDNVVCFTRHEPIGVCGAITPWNFPLLMLVWKLAPALCCGNTMVLKPAEQTPLTALYLGSLIKEAGFPPGVVNIVPGFGPTVGAAISSHPQINKIAFTGSTEVGKLVKEAASRSNLKRVTLELGGKNPCIVCADADLDLAVECAHQGVFFNQGQCCTAASRVFVEEQVYSEFVRRSVEYAKKRPVGDPFDVKTEQGPQIDQKQFDKILELIESGKKEGAKLECGGSAMEDKGLFIKPTVFSEVTDNMRIAKEEIFGPVQPILKFKSIEEVIKRANSTDYGLTAAVFTKNLDKALKLASALESGTVWINCYNALYAQAPFGGFKMSGNGRELGEYALAEYTEVKTVTIKLGDKNP

55-60 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filter solution of 20 mM Tris-HCl, 150 mM NaCl, 20% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Moretti A, et al. Crystal structure of human aldehyde dehydrogenase 1A3 complexed with NAD+ and retinoic acid. Sci Rep. 2016 Oct 19;6:35710.  [Content Brief]

  [2]. Duan JJ, et al. ALDH1A3, a metabolic target for cancer diagnosis and therapy. Int J Cancer. 2016 Sep 1;139(5):965-75.  [Content Brief]