AMIGO2 蛋白, Human (HEK293, His)

AMIGO2 Protein, Human (HEK293, His) is an 58-85 kDa AMIGO2 protein fused His-tag expressed in HEK 293 cells. AMIGO2 Protein, Human (HEK293, His) interacts with PDK1 to activate the Akt pathway localizated to the plasma membrane^[1].

AMIGO2 是一种调节 PDK1 膜定位和 Akt 激活的支架蛋白。该蛋白含有 6 个富含亮氨酸的重复序列 (LRR)、一个 IgG 样结构域、一个跨膜结构域和一个胞质结构域。AMIGO2 通过控制 PDK1 定位到质膜来发挥作用，然后直接与 PDK1 相互作用激活 Akt 通路^[1]。
内皮细胞 (EC) 中 AMIGO2 缺失会导致细胞凋亡Akt 失活抑制血管生成^[1]。
AMIGO2 对于调节 T 细胞功能很重要，Amigo2 过表达会抑制 NF-kB 转录活性，而 Amigo2 缺陷会增强 Akt，但会抑制GSK-3β 磷酸化并促进 Th 细胞中 NF-kB 和 NFAT1 的核转位^[1]。

Human

HEK293

C-6*His

Q86SJ2 (V40-A392)

347902  [NCBI]

VCPTACICATDIVSCTNKNLSKVPGNLFRLIKRLDLSYNRIGLLDSEWIPVSFAKLNTLILRHNNITSISTGSFSTTPNLKCLDLSSNKLKTVKNAVFQELKVLEVLLLYNNHISYLDPSAFGGLSQLQKLYLSGNFLTQFPMDLYVGRFKLAELMFLDVSYNRIPSMPMHHINLVPGKQLRGIYLHGNPFVCDCSLYSLLVFWYRRHFSSVMDFKNDYTCRLWSDSRHSRQVLLLQDSFMNCSDSIINGSFRALGFIHEAQVGERLMVHCDSKTGNANTDFIWVGPDNRLLEPDKEMENFYVFHNGSLVIESPRFEDAGVYSCIAMNKQRLLNETVDVTINVSNFTVSRSHAHHHHHHH

58-85 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PB, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Hyojin Park, et al.AMIGO2, a novel membrane anchor of PDK1, controls cell survival and angiogenesis via Akt activation. J Cell Biol  [Content Brief]

  [2]. Zhilin Li, et al. AMIGO2 modulates T cell functions and its deficiency in mice ameliorates experimental autoimmune encephalomyelitis. Brain Behav Immun. 2017 May;62:110-123.  [Content Brief]