1. 重组蛋白
  2. Receptor Proteins
  3. Cell Adhesion Molecules (CAMs)
  4. AMIGO2 蛋白, Human (HEK293, His)

AMIGO2 蛋白, Human (HEK293, His)

目录号: HY-P7495
COA 产品使用指南

AMIGO2 蛋白, Human (HEK293, His)是一个58-85 kDa 的融合 His 标签的 AMIGO2 蛋白,在 HEK 293 细胞中表达。AMIGO2 蛋白, Human (HEK293, His) 与 PDK1 相互作用,激活位于质膜上的 Akt 通路。

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规格 价格 是否有货 数量
10 μg ¥1250 In-stock
50 μg ¥3500 In-stock
100 μg   询价  

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描述

AMIGO2 Protein, Human (HEK293, His) is an 58-85 kDa AMIGO2 protein fused His-tag expressed in HEK 293 cells. AMIGO2 Protein, Human (HEK293, His) interacts with PDK1 to activate the Akt pathway localizated to the plasma membrane[1].

研究背景

AMIGO2 是一种调节 PDK1 膜定位和 Akt 激活的支架蛋白。该蛋白含有 6 个富含亮氨酸的重复序列 (LRR)、一个 IgG 样结构域、一个跨膜结构域和一个胞质结构域。AMIGO2 通过控制 PDK1 定位到质膜来发挥作用,然后直接与 PDK1 相互作用激活 Akt 通路[1]
内皮细胞 (EC) 中 AMIGO2 缺失会导致细胞凋亡Akt 失活抑制血管生成[1]
AMIGO2 对于调节 T 细胞功能很重要,Amigo2 过表达会抑制 NF-kB 转录活性,而 Amigo2 缺陷会增强 Akt,但会抑制GSK-3β 磷酸化并促进 Th 细胞中 NF-kB 和 NFAT1 的核转位[1]

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q86SJ2 (V40-A392)

基因 ID
蛋白结构
N-term
AMIGO2 (V40-A392)
Accession # Q86SJ2
6*His
C-term
同用名
rHuAmphoterin-Induced Protein 2, His; AMIGO2; Alivin-1; Amphoterin-Induced Protein 2
氨基酸序列

VCPTACICATDIVSCTNKNLSKVPGNLFRLIKRLDLSYNRIGLLDSEWIPVSFAKLNTLILRHNNITSISTGSFSTTPNLKCLDLSSNKLKTVKNAVFQELKVLEVLLLYNNHISYLDPSAFGGLSQLQKLYLSGNFLTQFPMDLYVGRFKLAELMFLDVSYNRIPSMPMHHINLVPGKQLRGIYLHGNPFVCDCSLYSLLVFWYRRHFSSVMDFKNDYTCRLWSDSRHSRQVLLLQDSFMNCSDSIINGSFRALGFIHEAQVGERLMVHCDSKTGNANTDFIWVGPDNRLLEPDKEMENFYVFHNGSLVIESPRFEDAGVYSCIAMNKQRLLNETVDVTINVSNFTVSRSHAHHHHHHH

分子量

58-85 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against PB, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

AMIGO2 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
AMIGO2 Protein, Human (HEK293, His)
目录号:
HY-P7495
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