Aminoacylase-1 蛋白, Mouse (HEK293, His)

The Aminoacylase-1 protein plays an important role in cellular processes as it catalyzes the hydrolysis of N-acetylated amino acids, promoting the conversion of these compounds into acetate and free amino acids. This enzymatic activity highlights the importance of Aminoacylase-1 in the metabolic pathways responsible for the breakdown of N-acetylated amino acids, helping to release essential amino acids and acetate as by-products. Aminoacylase-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Aminoacylase-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Aminoacylase-1 Protein, Mouse (HEK293, His) is 408 a.a., with molecular weight of ~55 kDa.

Aminoacylase-1 是一种催化 N-乙酰化氨基酸水解的酶，导致乙酸盐和游离氨基酸的产生。这种酶活性是氨基酸代谢过程的一部分，其中 N-乙酰化氨基酸被裂解，释放出相应的氨基酸和乙酸盐。Aminoacylase-1 在此水解反应中的作用有助于调节氨基酸水平，对于维持细胞稳态至关重要。这种酶在控制氨基酸利用的复杂代谢途径网络中发挥着关键作用，并参与蛋白质合成和能量生产等过程。

Measured by its ability to cleave N-acetyl-L-Methione (Ac-Met). The specific activity is 19928.580 pmol/min/µg.

Mouse

HEK293

C-His

Q99JW2 (M1-S408)

109652  [NCBI]

MTTKDPESEHPSVTLFRQYLRICTVQPNPDYGGAITFLEERARQLGLSCQKIEVVPGFVITVLTWPGTNPSLPSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGSQDMKSVSIQYLEAVRRLKSEGHRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDAFTVFYSERSPWWVRVTSTGKPGHASRFIEDTAAEKLHKVISSILAFREKERQRLQANPHLKEGAVTSVNLTKLEGGVAYNVVPATMSASFDFRVAPDVDMKAFEKQLQRWCQEAGEGVTFEFAQKFTEPRMTPTDDSDPWWAAFSGACKAMNLTLEPEIFPAATDSRYIRAVGIPALGFSPMNRTPVLLHDHNERLHEDIFLRGVDIYTGLLSALAS

Approximately 55 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.