Amphiregulin 蛋白, Human (HEK293)

Amphiregulin Protein, Human (HEK293, His) is an EGF receptor (EGFR) ligand, and essential for epithelial development in various organs.

双调蛋白是一种 EGF 受体 (EGFR) 配体，对于各种器官的上皮发育至关重要。双调蛋白被认为在肝损伤模型中充当保护因子。在博莱霉素诱导的肺病小鼠模型中，重组人双调蛋白可提高存活率，抑制炎症和纤维化程度以及肺组织中 TUNEL 阳性细胞的数量。重组人双调蛋白治疗可增强肺上皮细胞中 Akt 和 Erk 的激活^[1]。

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect ≤ 14.39 ng/mL, corresponding to a specific activity ≥6.950×10⁴ U/mg.

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  Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 14.39 ng/mL, corresponding to a specific activity is 6.950×10⁴ U/mg.

Human

HEK293

Tag Free

P15514 (S101-K198)

374  [NCBI]

SVRVEQVVKPPQNKTESENTSDKPKRKKKGGKNGKNRRNRKKKNPCNAEFQNFCIHGECKYIEHLEAVTCKCQQEYFGERCGEKSMKTHSMIDSSLSK

Approximately 15-23 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS or PBS, pH 7.4, 8% trehalose.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Fukumoto J, et al. Amphiregulin attenuates bleomycin-induced pneumopathy in mice. Am J Physiol Lung Cell Mol Physiol. 2010 Feb;298(2):L131-8.  [Content Brief]