1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. AMPK (gamma 1/beta 1/alpha 2) 蛋白, Human (Unactive, sf9, His)

AMPK (gamma 1/beta 1/alpha 2) 蛋白, Human (Unactive, sf9, His)

目录号: HY-P702978
产品使用指南

AMPK gamma 1 是 AMP 激活蛋白激酶 (AMPK) 的 AMP/ATP 结合亚基,是细胞能量代谢的重要调节因子。它被低 ATP 水平激活,增强能量产生,同时抑制生物合成过程和细胞生长。AMPK (gamma 1/beta 1/alpha 2) 蛋白, Human (Unactive, sf9, His) 是重组的 AMPK (γ1/β1/α2) 蛋白,由 Sf9 insect cells 表达,带有 His 标签。AMPK (gamma 1/beta 1/alpha 2) 蛋白, Human (Unactive, sf9, His)

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描述

AMPK gamma 1 is the AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK) and is an important regulator of cellular energy metabolism. It is activated by low ATP levels, enhancing energy production while inhibiting biosynthetic processes and cell growth. AMPK (gamma 1/beta 1/alpha 2) Protein, Human (Unactive, sf9, His) is the recombinant human-derived AMPK (gamma 1/beta 1/alpha 2), expressed by Sf9 insect cells , with His labeled tag. ,

研究背景

AMPK γ 1/β 1/α 1 异三聚体是 AMP 激活蛋白激酶 (AMPK) 的 AMP/ATP 结合亚基,AMPK 是细胞能量代谢的关键调节因子。当细胞内 ATP 水平降低时,AMPK 被激活,启动一系列事件,增强能量产生,同时抑制能量消耗过程,如蛋白质、碳水化合物和脂质生物合成,以及细胞生长和增殖。AMPK 的 γ 非催化亚基介导与 AMP、ADP 和 ATP 的结合,影响 AMPK 的激活或抑制。AMP 结合通过诱导磷酸化和防止去磷酸化以变构方式激活 α 催化亚基。ADP 刺激磷酸化,而 ATP 促进去磷酸化,使 AMPK 酶失活。除了在能量调节中的作用之外,AMPK 还通过重塑肌动蛋白细胞骨架(可能通过肌球蛋白的间接激活)充当细胞极性的调节剂。AMPK 的异三聚体组成包括 α 催化亚基(PRKAA1 或 PRKAA2)、β 亚基(PRKAB1 或 PRKAB2)和 γ 非催化亚基(PRKAG1、PRKAG2 或 PRKAG3),凸显了其复杂的调节机制。AMPK 进一步与 FNIP1 和 FNIP2 相互作用来调节其细胞功能。

种属

Human

表达系统

Sf9 insect cells

标签

His

蛋白编号

P54619-1(M1-P331)&Q9Y478 (M1-I270)&P54646 (M1-R552)

基因 ID

5563

同用名
Subunits A2: PRKAA2; AMPK; AMPK2; PRKAA Subunit B1: PRKAB1; AMPK; HAMPKb; MGC17785 Subunit G1: PRKAG1; AMPKG; MGC866
分子量

G1:40kDa; B1:38 kDa; A2:69 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

文件资料

AMPK (gamma 1/beta 1/alpha 2) Protein, Human (Unactive, sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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AMPK (gamma 1/beta 1/alpha 2) Protein, Human (Unactive, sf9, His)
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