ANGPTL3/Angiopoietin-like 3 蛋白, Human (Biotinylated, HEK293, His-Avi)

ANGPTL3/angiopoietin-like 3 protein is a hepatic factor that complexly regulates lipid and glucose metabolism.It stimulates plasma triglycerides by inhibiting lipoprotein lipase (LPL), inhibits TG clearance through PCSK6 and FURIN recruitment, is independent of proteolytic cleavage, and is not affected by GPIHBP1.ANGPTL3/Angiopoietin-like 3 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived ANGPTL3/Angiopoietin-like 3 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

ANGPTL3/血管生成素样 3 蛋白作为肝因子发挥作用，复杂地参与脂质和葡萄糖代谢的调节。ANGPTL3 被提议在响应食物摄入而将能量底物导向储存或氧化组织方面发挥作用，它通过抑制脂蛋白脂肪酶 (LPL) 活性来刺激血浆甘油三酯 (TG)，从而抑制 TG 清除。这种效果是通过将前蛋白转化酶 PCSK6 和 FURIN 募集至 LPL 来实现的，从而导致 LPL 从细胞表面裂解和解离。有趣的是，这种调节功能不需要 ANGPTL3 蛋白水解切割，由 N 末端结构域介导，并且不受 GPIHBP1 的影响。此外，ANGPTL3 还能抑制内皮脂肪酶，从而提高血浆中高密度脂蛋白 (HDL) 胆固醇和磷脂的水平。通过与脂肪细胞结合，它激活脂肪分解，释放游离脂肪酸和甘油。ANGPTL3 选择性抑制氧化组织中的 LPL，将极低密度脂蛋白 (VLDL)-TG 引导至白色脂肪组织 (WAT) 进行储存以响应食物，可能与 WAT 中循环的肝源性 ANGPTL8 和 ANGPTL4 表达相配合。它还有助于降低血浆低密度脂蛋白 (LDL) 胆固醇水平，与经典的 APOE 和 LDLR 途径无关。此外，ANGPTL3 可能会刺激下丘脑 LPL 活性，而在体外，它会抑制 LPL 活性，但对 GPIHBP1 稳定的 LPL 没有作用。

1. Immobilized Biotinylated Human ANGPTL3 His at 0.5 μg/mL (100 μL/Well). Dose response curve for Anti-ANGPTL3 Antibody hFc with the EC₅₀ ≤ 11.5 ng/mL determined by ELISA.
2. Immobilized Anti-ANGPTL3 Antibody at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Human ANGPTL3, His Tag with the EC₅₀ ≤ 5.3 ng/mL determined by ELISA.

Human

HEK293

C-Avi;C-His

Q9Y5C1 (S17-K219)

27329  [NCBI]

30-38 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.