Animal-Free BMP-7 蛋白, Human (His)

BMP-7 protein is an important member of the TGF-β superfamily and is critical in embryogenesis, hematopoiesis, neurogenesis and bone morphogenesis. It activates canonical BMP signaling through ACVR1 and ACVR2A, phosphorylates and activates ACVR1, which in turn phosphorylates SMAD1/5/8 for target gene transcriptional regulation. Animal-Free BMP-7 Protein, Human (His) is the recombinant human-derived animal-FreeBMP-7 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free BMP-7 Protein, Human (His) is 117 a.a., with molecular weight of ~14.00 kDa.

BMP-7 蛋白是 TGF-β 超家族的重要成员，在各种生物过程中发挥着至关重要的作用，包括胚胎发生、造血、神经发生和骨骼形态发生。它通过与 I 型受体 ACVR1 和 II 型受体 ACVR2A 结合启动典型的 BMP 信号级联，导致 ACVR1 磷酸化和激活。随后，ACVR1 磷酸化 SMAD1/5/8，后者作为细胞核中的激活子和阻遏子调节靶基因转录。在特定功能中，例如发育中的脊髓神经元中的生长锥塌陷和单核细胞趋化性，BMP-7 利用 BMPR2 作为额外的 II 型受体。除了经典途径之外，BMP-7还通过非经典途径（例如P38 MAP激酶信号级联）发出信号，通过激活靶基因（包括转录因子SOX家族的成员）来促进棕色脂肪细胞分化。BMP-7 进一步调节 HAMP 的表达，该过程受到其与 ERFE 相互作用的抑制。这种同二聚体蛋白形成二硫键连接的复合物，并与各种蛋白相互作用，包括 SOSTDC1、TWSG1、FBN1、FBN2、ACVR1、ACVR2A、NOG、SCUBE3 和 ERFE，每种蛋白都有助于其多样化的功能。

Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <0.65 μg/mL.

Human

E. coli

C-His

P18075 (M315-H431)

655  [NCBI]

MANVAENSSSDQRQACKKHELYVSFRDLGWQDWIIAPEGYAAYYCEGECAFPLNSYMNATNHAIVQTLVHFINPETVPKPCCAPTQLNAISVLYFDDSSNVILKKYRNMVVRACGCH

Approximately 14.00 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

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