Animal-Free EGF 蛋白, Pig (His)

The EGF protein acts as a potent stimulator of growth in a variety of epidermal and epithelial tissues in both in vivo and in vitro settings and exhibits growth-promoting effects on certain fibroblasts in cell culture. Animal-Free EGF Protein, Pig (His) is the recombinant pig-derived animal-FreeEGF protein, expressed by E. coli , with C-His labeled tag.

EGF 蛋白在体内和体外环境中均可作为多种表皮和上皮组织生长的有效刺激剂，并且对细胞培养物中的某些成纤维细胞表现出生长促进作用。除了在组织生长中的作用外，该蛋白还充当促镁激素，通过与 EGFR 结合并激活镁通道 TRPM6，积极促进肾远曲小管中的镁重吸收，如相似性所示。此外，EGF 蛋白参与分子相互作用，例如与 EGFR 结合、促进 EGFR 二聚化以及与 RHBDF1 相互作用。后一种相互作用可能有助于内质网中EGF 的潜在保留，通过内质网相关降解 (ERAD) 调节其降解。此外，它与 RHBDF2 的相互作用暗示了其在细胞过程中的多方面作用，进一步凸显了EGF 蛋白在各种分子途径中的多功能性。

Pig

E. coli

C-His

Q00968 (N970-R1022)

397083  [NCBI]

MNSYSECPPSHDGYCLHGGVCMYIEAVDSYACNCVFGYVGERCQHRDLKWWELR

Approximately 7.1 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a solution containing 1X PBS, pH 8.0.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.