Animal-Free FGF-21 蛋白, Human (His)

FGF-21 Proteinas, pivotal in promoting glucose uptake in adipocytes, induces SLC2A1/GLUT1 expression, not affecting SLC2A4/GLUT4, contingent on KLB.It significantly regulates systemic glucose homeostasis and insulin sensitivity, with direct KLB interaction via its C-terminus.Engagement with FGFR4 adds complexity to its molecular mechanisms, highlighting its role in orchestrating cellular responses beyond localized effects.Animal-Free FGF-21 Protein, Human (His) is the recombinant human-derived animal-FreeFGF-21 protein, expressed by E.coli , with C-His labeled tag.

FGF-21 蛋白通过特异性诱导葡萄糖转运蛋白 SLC2A1/GLUT1 的表达，同时不影响 SLC2A4/GLUT4 的表达，在促进分化脂肪细胞内的葡萄糖摄取方面发挥关键作用。其活动取决于 KLB 的存在。除了其局部作用外，这种蛋白质还对全身葡萄糖稳态和胰岛素敏感性的调节做出了重大贡献。通过其 C 末端促进与 KLB 的直接相互作用，强调了其功能的分子基础。此外，该蛋白质与 FGFR4 结合，进一步凸显了其在协调细胞反应中相互作用的复杂性。

Measure by its ability to induce proliferation in BaF3 cells transfected with human FGFRIIIc. The ED₅₀ for this effect is <0.4 μg/mL.

Human

E. coli

C-His

Q9NSA1 (H29-S209)

26291  [NCBI]

MHPIPDSSPLLQFGGQVRQRYLYTDDAQQTEAHLEIREDGTVGGAADQSPESLLQLKALKPGVIQILGVKTSRFLCQRPDGALYGSLHFDPEACSFRELLLEDGYNVYQSEAHGLPLHLPGNKSPHRDPAPRGPARFLPLPGLPPALPEPPGILAPQPPDVGSSDPLSMVGPSQGRSPSYAS

Approximately 20.35 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.