Animal-Free GM-CSF 蛋白, Human (His)

The GM-CSF protein functions as a key cytokine that promotes the growth and differentiation of hematopoietic precursor cells of different lineages, such as granulocytes, macrophages, eosinophils, and erythrocytes. In its monomeric form, GM-CSF acts as a signaling molecule, orchestrating complex receptor assemblies. Animal-Free GM-CSF Protein, Human (His) is the recombinant human-derived animal-FreeGM-CSF protein, expressed by E. coli , with N-His labeled tag. The total length of Animal-Free GM-CSF Protein, Human (His) is 127 a.a., with molecular weight of ~15.4 kDa.

GMP GM-CSF 蛋白作为关键细胞因子发挥作用，促进不同谱系的造血前体细胞（如粒细胞、巨噬细胞、嗜酸性粒细胞和红细胞）的生长和分化。GMP GM-CSF 以其单体形式充当信号分子，协调复杂的受体组装。该受体复合物呈十二聚体形状，由两个头对头的六聚体组成，每个六聚体由两个α、两个β和两个配体亚基组成。这种结构的复杂性强调了 GMP GM-CSF 在指导造血系统内细胞反应中的特异性和调节作用。

Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <80 pg/mL. The specific activity of recombinant human GM-CSF is approximately >1 x 10⁷ IU/mg.

Human

E. coli

N-His

P04141 (A18-E144)

1437  [NCBI]

APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE

Approximately 15.4 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a solution containing 1X PBS, pH 8.0.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.