Animal-Free GRO-alpha/CXCL1 蛋白, Human (His)

GRO-alpha/CXCL1 protein attracts neutrophils and potentially contributes to inflammation through autocrine effects on endothelial cells. Processed forms of GRO-alpha, including GRO-alpha(4-73), GRO-alpha(5-73), and GRO-alpha(6-73), exhibit 30-fold greater chemotactic activity than the full-length protein. Animal-Free GRO-alpha/CXCL1 Protein, Human (His) is the recombinant human-derived animal-FreeGRO-alpha/CXCL1 protein, expressed by E. coli , with N-His labeled tag. The total length of Animal-Free GRO-alpha/CXCL1 Protein, Human (His) is 73 a.a., with molecular weight of ~8.67 kDa.

GRO-α/CXCL1 蛋白对中性粒细胞表现出趋化活性，并可能在炎症中发挥作用，以自分泌方式对内皮细胞发挥作用。体外研究表明，GRO-α 的加工形式，即 GRO-α(4-73)、GRO-α(5-73) 和 GRO-α(6-73)，显示出显着增强的趋化活性，为 30与全长蛋白质相比高倍。

Measure by its ability to chemoattract BaF3 cells transfected with human CXCR2. The ED₅₀ for this effect is <3 ng/mL.

Human

E. coli

N-His

P09341 (A35-N107)

2919  [NCBI]

ASVATELRCQCLQTLQGIHPKNIQSVNVKSPGPHCAQTEVIATLKNGRKACLNPASPIVKKIIEKMLNSDKSN

Approximately 8.67 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.