Animal-Free HDGF 蛋白, Human (His)

The HDGF protein acts as a transcriptional repressor and was previously thought to have mitogenic activity for fibroblasts and be a heparin-binding protein. However, recent studies have shown that it does not exhibit mitogenic activity for fibroblasts and does not bind heparin. Animal-Free HDGF Protein, Human (His) is the recombinant human-derived animal-FreeHDGF protein, expressed by E. coli , with C-His labeled tag.

肝细胞瘤衍生生长因子 (HDGF) 具有转录抑制因子和成纤维细胞有丝分裂因子的双重作用。虽然它作为转录调节的阻遏物，影响细胞过程，但它也表现出促有丝分裂活性，特别是对于成纤维细胞。HDGF 已被鉴定为肝素结合蛋白，这表明它参与与肝素的相互作用并可能调节细胞反应。然而，值得注意的是，存在相互矛盾的报道，一些研究表明 HDGF 缺乏成纤维细胞有丝分裂活性，并且不与肝素结合。这些发现强调了 HDGF 功能的复杂性，展示了其在转录控制、有丝分裂信号传导以及与肝素的潜在相互作用中的不同作用。

Human

E. coli

C-His

P51858-1 (M1-L240)

3068  [NCBI]

MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCVEEPEPEPEAAEGDGDKKGNAEGSSDEEGKLVIDEPAKEKNEKGALKRRAGDLLEDSPKRPKEAENPEGEEKEAATLEVERPLPMEVEKNSTPSEPGSGRGPPQEEEEEEDEEEEATKEDAEAPGIRDHESL

Approximately 29.60 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.