Animal-Free IL-1 beta 蛋白, Mouse (His)

IL-1 beta protein is a potent pro-inflammatory cytokine and major endogenous pyrogen that induces immune cell effects such as prostaglandin synthesis, neutrophil activation, T- and B-cell activation, and fibroblast proliferation . It promotes Th17 differentiation and synergizes with IL12 to promote Th1 IFNG synthesis. Animal-Free IL-1 beta Protein, Mouse (His) is the recombinant mouse-derived animal-FreeIL-1 beta protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-1 beta Protein, Mouse (His) is 152 a.a., with molecular weight of ~18.3 kDa.

IL-1 β 蛋白是一种有效的促炎细胞因子，最初被发现是主要的内源性热原。它对免疫细胞具有多种作用，如诱导前列腺素合成、激活中性粒细胞、促进T细胞活化和细胞因子产生、刺激B细胞活化和抗体产生、增加成纤维细胞增殖和胶原蛋白产生。IL-1β蛋白还具有促进Th17细胞分化的作用，并与IL12协同作用，诱导Th1细胞合成IFNG。此外，它还通过与 TNF 和 IL6 协同诱导 VEGF 的产生来促进血管生成。IL-1 β 蛋白参与细胞焦亡下游炎症的转导，其成熟形式通过gasdermin-D (GSDMD) 孔特异性释放到细胞外环境中。它以单体形式存在，并与 MEFV 以及 IL1B 信号转导所需的整合素 ITGAV:ITGBV 和 ITGA5:ITGB1 相互作用。IL-1 beta 蛋白还与货物受体 TMED10、HSP90AB1 和 HSP90B1 相互作用，从而促进货物易位到 ERGIC 中。

Measure by its ability to induce D10.G4.1 cells proliferation. The ED₅₀ for this effect is< 8 pg/mL. The specific activity of recombinan tmouse IL-1 beta is approximately >1.2x 10⁸ IU/mg/.

Mouse

E. coli

C-His

P10749 (V118-S269)

16176  [NCBI]

MVPIRQLHYRLRDEQQKSLVLSDPYELKALHLNGQNINQQVIFSMSFVQGEPSNDKIPVALGLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVFNKIEVKSKVEFESAEFPNWYISTSQAEHKPVFLGNNSGQDIIDFTMESVSS

Approximately 18.3 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.