Animal-Free IL-11 蛋白, Human (His)

IL-11 protein is a cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, leading to increased platelet production. Animal-Free IL-11 Protein, Human (His) is the recombinant human-derived animal-FreeIL-11 protein, expressed by E. coli , with N-His labeled tag. The total length of Animal-Free IL-11 Protein, Human (His) is 178 a.a., with molecular weight of ~19.95 kDa.

IL-11 蛋白作为一种多方面的细胞因子出现，协调重要的生物过程。它刺激造血干细胞和巨核细胞祖细胞的增殖，最终导致巨核细胞的成熟和增加血小板的产生。此外，IL-11 通过促进肝细胞增殖以应对损伤，从而在肝再生中发挥关键作用。它与由 IL6ST 和 IL11RA 组成的受体复合物结合，启动信号级联反应，促进细胞增殖。这种相互作用激活细胞内蛋白激酶并触发 STAT3 的磷酸化。值得注意的是，IL-11 在信号传导方式上表现出多功能性：它在与膜结合的 IL11RA 和 IL6ST 相互作用时参与“经典信号传导”，或者，当与可溶性 IL11RA 结合到 IL6ST 时，它参与“反式信号传导”。IL-11 及其受体 IL11RA 和 IL6ST 之间错综复杂的相互作用，形成了一个多聚体信号复合物，对多种生理反应具有深远的影响。

Measure by its ability to induce T11 cells proliferation. The ED₅₀ for this effect is <0.2 ng/mL. The specific activity of recombinant human IL-11 is approximately >1 x 10⁷ IU/mg.

Human

E. coli

N-His

P20809 (P22-L199)

3589  [NCBI]

PGPPPGPPRVSPDPRAELDSTVLLTRSLLADTRQLAAQLRDKFPADGDHNLDSLPTLAMSAGALGALQLPGVLTRLRADLLSYLRHVQWLRRAGGSSLKTLEPELGTLQARLDRLLRRLQLLMSRLALPQPPPDPPAPPLAPPSSAWGGIRAAHAILGGLHLTLDWAVRGLLLLKTRL

Approximately 19.95 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.