Animal-Free IL-21 蛋白, Human (His)

IL-21 protein is an immunomodulatory cytokine that promotes the transition from innate immunity to adaptive immunity. It induces B cells to produce IgG(1) and IgG(3), harnessing an effective antibody response to fight viral infections. Animal-Free IL-21 Protein, Human (His) is the recombinant human-derived animal-FreeIL-21 protein, expressed by E. coli , with C-His labeled tag.

IL-21 蛋白是一种具有免疫调节活性的细胞因子，是协调先天免疫和适应性免疫之间相互作用的关键介质。值得注意的是，它会诱导 B 细胞产生 IgG(1) 和 IgG(3)，强调其在形成体液免疫反应中的作用。此外，IL-21 与滤泡辅助 T (Tfh) 细胞的生成和维持密切相关，在生发中心的形成（适应性免疫的重要过程）中发挥着至关重要的作用。它与 IL6 合作，有助于 Tfh 细胞的早期生成，这对于在急性病毒感染期间建立有效的抗体反应至关重要。此外，IL-21 可能在自然杀伤 (NK) 细胞的增殖和成熟中发挥作用，特别是与 IL15 协同作用。其调节影响延伸至成熟的 B 细胞和 T 细胞，调节其增殖以响应激活刺激。IL-21 与 IL15 和 IL18 协同作用，刺激 T 细胞和 NK 细胞产生干扰素 γ，突出其对免疫反应的多方面影响。在 T 细胞介导的免疫反应过程中，IL-21 表现出抑制树突状细胞 (DC) 激活和成熟的潜力，为其调节功能增添了另一层。

Measure by its ability to enhance IFN gamma secretion in NK-92 cells. The ED₅₀ for this effect is <10 ng/mL.

Human

E. coli

C-His

Q9HBE4-1 (Q32-S162)

59067  [NCBI]

MQDRHMIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVETNCEWSAFSCFQKAQLKSANTGNNERIINVSIKKLKRKPPSTNAGRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKMIHQHLSSRTHGSEDS

Approximately 16.2 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.