2. 重组蛋白
  3. Cytokines and Growth Factors Animal-free Recombinant Proteins
  4. CSF & Receptors
  5. Multi-CSF/IL-3
  6. Animal-Free IL-3 蛋白, Human (His)

Animal-Free IL-3 蛋白, Human (His)

目录号: HY-P70576AF
COA 产品使用指南

Animal-Free IL-3 蛋白, Human (His) 是重组的 无动物源 IL-3 蛋白，由 E. coli 表达，带有 C-His 标签。Animal-Free IL-3 蛋白, Human (His) ，分子量约为 ~16 kDa。

无动物成分 (Animal-free) 重组蛋白，即生产过程中不使用或接触任何动物来源的材料和试剂，具有高一致性和稳定性。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
Free Sample   Apply now  
2 μg ￥500 In-stock
10 μg ￥1500 In-stock
50 μg ￥4400 In-stock
100 μg   询价  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Animal-Free IL-3 Protein, Human (His) is the recombinant human-derived animal-FreeIL-3 protein, expressed by E. coli , with C-His labeled tag. , has molecular weight of ~16 kDa.
生物活性

Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is <0.15 ng/mL. The specific activity of recombinant human IL-3 is approximately >1.2 x 106 IU/mg
种属

Human
表达系统

E. coli
标签

C-His
蛋白编号

AAC08706.1 (A20-F152)
基因 ID

3562
蛋白结构
N-term

Accession # ATV93543.1
His IL-3
C-term
同用名
rHuIL-3; Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor
氨基酸序列

MAPMTQTTSLKTSWVNCSNMIDEIITHLKQPPLPLLDFNNLNGEDQDILMENNLRRPNLEAFNRAVKSLQNASAIESILKNLLPCLPLATAAPTRHPIHIKDGDWNEFRRKLTFYLKTLENAQAQQTTLSLAIF

分子量

Approximately 16 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.
组分

Lyophilized from a solution containing 1X PBS, pH 8.0, trehalose.
内毒素含量

<0.1 EU per 1 μg of the protein by the LAL method.
复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
运输条件

Room temperature in continental US; may vary elsewhere.
文件资料

COA (209 KB)

产品使用指南 (1213 KB)

Animal-Free IL-3 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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