Animal-Free IL-36 gamma/IL-1F9 蛋白, Human (His)

IL-36 gamma/IL-1F9 protein activates the NF-kappa-B and MAPK pathways, induces the expression of various chemokines and pro-inflammatory factors, and promotes local inflammatory responses in the epithelial barrier. It affects keratinocytes, dendritic cells, and T cells, driving tissue infiltration, maturation, and proliferation. Animal-Free IL-36 gamma/IL-1F9 Protein, Human (His) is the recombinant human-derived animal-FreeIL-36 gamma/IL-1F9 protein, expressed by E. coli , with C-His, C-His labeled tag.

IL-36 gamma/IL-1F9 蛋白是一种细胞因子，与 IL1RL2/IL-36R 受体结合并通过其发出信号，激活靶细胞中的 NF-kappa-B 和 MAPK 信号通路，从而促进促炎反应。作为被认为存在于上皮屏障中的 IL-36 信号系统的重要组成部分，IL-36 γ 与 IL-1 系统具有相似之处，并利用辅助受体 IL1RAP。它似乎是皮肤炎症反应不可或缺的一部分，对角质形成细胞、树突状细胞产生影响，并间接影响 T 细胞，从而驱动组织浸润、细胞成熟和增殖。在培养的角质形成细胞中，IL-36 γ 诱导多种趋化因子的表达，包括 CCL3、CCL4、CCL5、CCL2、CCL17、CCL22、CL20、CCL5、CCL2、CCL17、CCL22、CXCL8、CCL20 和 CXCL1。此外，IL-36 γ 刺激其自身表达以及原型皮肤促炎参数（例如 TNF-α、S100A7/牛皮癣和诱导型 NOS）的表达。它可能在特定中性粒细胞气道炎症期间的促炎反应中发挥作用，激活原代肺成纤维细胞中的丝裂原激活蛋白激酶和 NF-κ B，并刺激肺成纤维细胞中 IL-8、CXCL3 和 Th17 趋化因子 CCL20 的表达。IL-36 γ 也可能参与对真菌病原体（例如烟曲霉）的先天免疫反应。IL-36 γ 与货物受体 TMED10 相互作用，从细胞质易位至内质网-高尔基中间室 (ERGIC)，促进分泌。

Measure by its ability to induce IL-8 secretion in A431 cells. The ED₅₀ for this effect is <5 ng/mL.

Human

E. coli

C-His;C-His

Q9NZH8-1 (S18-D169)

56300  [NCBI]

MSMCKPITGTINDLNQQVWTLQGQNLVAVPRSDSVTPVTVAVITCKYPEALEQGRGDPIYLGIQNPEMCLYCEKVGEQPTLQLKEQKIMDLYGQPEPVKPFLFYRAKTGRTSTLESVAFPDWFIASSKRDQPIILTSELGKSYNTAFELNIND

Approximately 17.98 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.