Animal-Free IL-6 蛋白, Human (His)

IL-6 protein is a multifunctional cytokine that plays multiple biological functions in immunity, tissue regeneration, and metabolism. After binding to IL6R, the resulting complex binds to the signaling subunit IL6ST/gp130, triggering the intracellular IL6 signaling pathway. Animal-Free IL-6 Protein, Human (His) is the recombinant human-derived animal-FreeIL-6 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-6 Protein, Human (His) is 184 a.a., with molecular weight of ~21.8 kDa.

GMP IL-6 蛋白是一种多功能细胞因子，在免疫、组织再生和代谢中发挥多种生物学功能。与 IL6R 结合后，产生的复合物与信号亚基 IL6ST/gp130 结合，触发细胞内 IL6 信号通路。它与膜结合的 IL6R 和 IL6ST 的相互作用刺激“经典信号传导”，而 IL6 和可溶性 IL6R 与 IL6ST 的结合则诱导“反式信号传导”。此外，当发射细胞上的膜结合 IL6:IL6R 复合物激活邻近接收细胞上的 IL6ST 受体时，就会发生“簇信号传导”。IL-6 作为急性期反应的有效诱导剂，在感染和组织损伤期间快速动员宿主防御，尽管过度的 IL6 合成与疾病病理有关。在先天免疫反应中，IL-6 由骨髓细胞（例如巨噬细胞和树突状细胞）在感染或组织损伤部位通过 Toll 样受体识别病原体后合成。在适应性免疫反应中，IL-6对于B细胞分化为免疫球蛋白分泌细胞至关重要，并且在CD4(+) T细胞亚群的分化中发挥重要作用。它是生发中心形成所必需的滤泡辅助 T (Tfh) 细胞发育的关键因素，并且是驱动幼稚 CD4(+) T 细胞向 Th17 谱系所必需的。此外，IL-6 对于骨髓瘤细胞的增殖和浆母细胞的存活至关重要。

1.Measure by its ability to induce proliferation in TF-1 cells The ED₅₀ for this effect is <0.5 ng/mL. The specific activity of recombinant human IL-6 is approximately >5 x 10⁸ IU/mg.
2.Measure by its ability to induce proliferation in MCF-7 cells. The ED₅₀ for this effect is <4.4 ng/mL.

Human

E. coli

C-His

P05231 (P29-M212)

3569  [NCBI]

MVPPGEDSKDVAAPHRQPLTSSERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALRQM

Approximately 21.8 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.