Animal-Free IL-8/CXCL8 蛋白, Human (His)

IL-8/CXCL8 protein, a vital chemotactic factor, orchestrates inflammatory responses by attracting neutrophils, basophils, and T-cells to clear pathogens. It activates neutrophils and binds to CXCR1/CXCR2 receptors, initiating downstream signaling pathways. IL-8/CXCL8 homodimerizes, disrupted by tick evasin-3, and interacts with TNFAIP6, potentially regulating chemokine activity in the inflammatory microenvironment. Animal-Free IL-8/CXCL8 Protein, Human (His) is the recombinant human-derived animal-FreeIL-8/CXCL8 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free IL-8/CXCL8 Protein, Human (His) is 72 a.a., with molecular weight of ~9.32 kDa.

IL-8/CXCL8蛋白作为关键的趋化因子，通过吸引中性粒细胞、嗜碱性粒细胞和T细胞来有效清除病原体并保护宿主免受感染，从而在介导炎症反应中发挥核心作用。它还对中性粒细胞激活有显着贡献。IL-8/CXCL8 响应炎症刺激而释放，通过与 G 蛋白偶联受体 CXCR1 和 CXCR2（主要存在于中性粒细胞、单核细胞和内皮细胞中）结合来发挥作用。G 蛋白异源三聚体（α、β、γ 亚基）与 CXCR1/CXCR2 受体组成型结合，IL-8 激活导致 Gα（中性粒细胞中的 GNAI2）释放 β 和 γ 亚基，随后激活下游信号通路，包括 PI3K 和 MAPK 通路。IL-8/CXCL8 形成同型二聚体，这种二聚体被蜱 evasin-3 破坏。此外，IL-8/CXCL8 通过其 Link 结构域与 TNFAIP6 相互作用，这种相互作用干扰趋化因子与糖胺聚糖的结合，表明其在调节炎症微环境中趋化因子活性中发挥调节作用。

Measure by its ability to chemoattract PBMC. The ED₅₀ for this effect is <2 ng/mL

Human

E. coli

C-His

P10145 (S28-S99)

3576  [NCBI]

MSAKELRCQCIKTYSKPFHPKFIKELRVIESGPHCANTEIIVKLSDGRELCLDPKENWVQRVVEKFLKRAENS

Approximately 9.32 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.