Animal-Free MIF 蛋白, Human (His)

The MIF protein is a pro-inflammatory cytokine that is critical for the innate immune response against bacterial pathogens. Its presence at sites of inflammation suggests a role in modulating macrophage function to promote host defense. Animal-Free MIF Protein, Human (His) is the recombinant human-derived animal-FreeMIF protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free MIF Protein, Human (His) is 115 a.a., with molecular weight of ~13.28 kDa.

MIF 蛋白是一种促炎细胞因子，在针对细菌病原体的先天免疫反应中发挥着至关重要的作用。它在炎症部位的表达表明它参与调节宿主防御中的巨噬细胞功能。MIF 抵消糖皮质激素的抗炎作用。尽管MIF在体外具有苯丙酮酸互变异构酶和多巴色素互变异构酶活性，但MIF的生理底物仍不清楚。目前尚不清楚互变异构酶活性是否与其细胞因子活性相关。

Human

E. coli

C-His

P14174 (M1-A115)

4282  [NCBI]

MPMFIVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQLMAFGGSSEPCALCSLHSIGKIGGAQNRSYSKLLCGLLAERLRISPDRVYINYYDMNAANVGWNNSTFA

Approximately 13.28 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.