1. 重组蛋白
  2. Cytokines and Growth Factors Animal-free Recombinant Proteins
  3. Chemokine & Receptors
  4. CC Chemokines
  5. MIP-1 alpha/CCL3
  6. Animal-Free MIP-1 alpha/CCL3 蛋白, Mouse (His)

Animal-Free MIP-1 alpha/CCL3 蛋白, Mouse (His)

目录号: HY-P7768AF
COA 产品使用指南 技术支持

MIP-1 α/CCL3 蛋白是一种单核因子,具有炎症、致热和趋化动力学特性。它在嗜酸性粒细胞中诱导有效的趋化性,并通过高亲和力受体结合激活中性粒细胞中的钙释放。Animal-Free MIP-1 alpha/CCL3 蛋白, Mouse (His) 是重组的 无动物源 MIP-1 α/CCL3 蛋白,由 E. coli 表达,带有 N-His 标签。产品推荐用于细胞培养。

无动物成分 (Animal-free) 重组蛋白,即生产过程中不使用或接触任何动物来源的材料和试剂,具有高一致性和稳定性。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample   Apply now  
2 μg ¥710 In-stock
10 μg ¥1980 In-stock
50 μg ¥5560 In-stock
100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

MIP-1 alpha/CCL3 protein, a monokine, displays inflammatory, pyrogenic, and chemokinetic properties.It induces potent chemotaxis in eosinophils and activates calcium release in neutrophils via high-affinity receptor binding.Animal-Free MIP-1 alpha/CCL3 Protein, Mouse (His) is the recombinant mouse-derived animal-FreeMIP-1 alpha/CCL3 protein, expressed by E.coli , with N-His, N-His labeled tag.This product is for cell culture use only.

研究背景

MIP-1 α/CCL3 蛋白是一种单因子,具有炎症、致热和趋化动力学特性。它对嗜酸性粒细胞表现出有效的趋化活性,并在与高亲和力受体结合后触发中性粒细胞中的钙释放。

生物活性

Measure by its ability to chemoattract BaF3 cells transfected with human CCR5. The ED50 for this effect is <1.8 ng/mL.

种属

Mouse

表达系统

E. coli

标签

N-His;N-His

蛋白编号

P10855 (A24-A92)

基因 ID
蛋白结构
N-term
His
CCL3 (A24-A92)
Accession # P10855
C-term
中文名
无动物源成分 MIP-1 alpha 蛋白/CC 趋化因子配体 3 蛋白
同用名
MIP-1a: Macrophage Inflammatory Protein-1α; LD78α
氨基酸序列

APYGADTPTACCFSYSRKIPRQFIVDYFETSSLCSQPGVIFLTKRNRQICADSKETWVQEYITDLELNA

分子量

Approximately 8.69 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

内毒素含量

<0.1 EU per 1 μg of the protein by the LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Animal-Free MIP-1 alpha/CCL3 Protein, Mouse (His)
目录号:
HY-P7768AF
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