Animal-Free TGF alpha/TGFA 蛋白, Human (His)

TGF α/TGFA protein is a mitogenic polypeptide that binds to EGFR and acts synergistically with TGF β to promote anchorage-dependent cell proliferation. Its interaction with MAGI3, SDCBP, and the SNTA1 PDZ domain, especially with SDCBP, is critical for efficient cell surface targeting. Animal-Free TGF alpha/TGFA Protein, Human (His) is the recombinant human-derived animal-FreeTGF alpha/TGFA protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free TGF alpha/TGFA Protein, Human (His) is 50 a.a., with molecular weight of ~6.49 kDa.

TGF α/TGFA 蛋白是一种促有丝分裂多肽，具有与 EGF 受体/EGFR 结合的能力，并与 TGF β 协同作用，促进软琼脂中非贴壁依赖性细胞增殖。该蛋白与 MAGI3、SDCBP 和 SNTA1 的 PDZ 结构域相互作用，与 SDCBP 的关联对于有效靶向细胞表面至关重要。内质网内未成熟的 TGF α/TGFA 与 CNIH 相互作用，其特征是前段且缺乏完整的 N-糖基化。此外，在高尔基体中，它可能与CNIH和GORASP2形成复合物。此外，通过其细胞质 C 端结构域，TGF α/TGFA 与 NKD2 相互作用，从而在不同的细胞区室和相互作用中表现出一系列潜在的功能作用。

Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <0.2 ng/mL. The specific activity of recombinant human TGF alpha is > 5 x 10⁶ IU/mg.

Human

E. coli

C-His

P01135 (V40-A89)

7039  [NCBI]

MVVSHFNDCPDSHTQFCFHGTCRFLVQEDKPACVCHSGYVGARCEHADLLA

Approximately 6.49 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 8.0, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.