1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens Animal-free Recombinant Proteins
  3. TNF Superfamily NK Cell CD Proteins
  4. TNF Superfamily Ligands TRAIL/CD253
  5. TRAIL/CD253
  6. Animal-Free TRAIL/TNFSF10 蛋白, Mouse (His)

Animal-Free TRAIL/TNFSF10 蛋白, Mouse (His)

目录号: HY-P7089AF
COA 产品使用指南

TRAIL/TNFSF10 蛋白与 TNFRSF10A、TNFRSF10B、TNFRSF10C、TNFRSF10D 和可能的 TNFRSF11B 结合,诱导细胞凋亡。它可以通过诱饵受体 TNFRSF10C、TNFRSF10D 和 TNFRSF11B 进行调节,这些受体不会诱导细胞凋亡。TRAIL/TNFSF10 作为同源三聚体存在,与其受体单体相互作用。这种复杂的分子相互作用控制着细胞凋亡信号通路。Animal-Free TRAIL/TNFSF10 蛋白, Mouse (His) 是重组的 无动物源 TRAIL/TNFSF10 蛋白,由 E. coli 表达,带有 C-His 标签。

无动物成分 (Animal-free) 重组蛋白,即生产过程中不使用或接触任何动物来源的材料和试剂,具有高一致性和稳定性。

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SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample   Apply now  
2 μg ¥780 In-stock
10 μg ¥2180 In-stock
50 μg ¥6100 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

TRAIL/TNFSF10 protein binds to TNFRSF10A, TNFRSF10B, TNFRSF10C, TNFRSF10D, and possibly TNFRSF11B, inducing apoptosis.It can be modulated by decoy receptors TNFRSF10C, TNFRSF10D, and TNFRSF11B, which do not induce apoptosis.TRAIL/TNFSF10 exists as a homotrimer, interacting with its receptor monomers.This complex molecular interaction governs apoptotic signaling pathways.Animal-Free TRAIL/TNFSF10 Protein, Mouse (His) is the recombinant mouse-derived animal-FreeTRAIL/TNFSF10 protein, expressed by E.coli , with C-His labeled tag.

研究背景

TRAIL/TNFSF10 蛋白是一种细胞因子,可与 TNFRSF10A/TRAILR1、TNFRSF10B/TRAILR2、TNFRSF10C/TRAILR3、TNFRSF10D/TRAILR4 以及可能的 TNFRSF11B/OPG 结合,诱导细胞凋亡。其凋亡活性可通过与诱饵受体 TNFRSF10C/TRAILR3、TNFRSF10D/TRAILR4 和 TNFRSF11B/OPG 结合来调节,这些受体缺乏诱导凋亡的能力。TRAIL/TNFSF10 作为同源三聚体存在,其中一个 TRAIL/TNFSF10 同源三聚体与三个 TNFRSF10A 单体相互作用,另一个同源三聚体与三个 TNFRSF10B 单体相互作用。这种多价相互作用强调了控制 TRAIL/TNFSF10 及其受体介导的细胞凋亡信号通路的复杂分子相互作用。

生物活性

Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is <1 ng/mL.

种属

Mouse

表达系统

E. coli

标签

C-His

蛋白编号

P50592 (P118-N291)

基因 ID
蛋白结构
N-term
TRAIL (P118-N291)
Accession # P50592
His
C-term
同用名
rMuTRAIL/TNFSF10; TNF-related apoptosis-inducing ligand; Tumor necrosis factor ligand superfamily member 10
氨基酸序列

MPRGGRPQKVAAHITGITRRSNSALIPISKDGKTLGQKIESWESSRKGHSFLNHVLFRNGELVIEQEGLYYIYSQTYFRFQEAEDASKMVSKDKVRTKQLVQYIYKYTSYPDPIVLMKSARNSCWSRDAEYGLYSIYQGGLFELKKNDRIFVSVTNEHLMDLDQEASFFGAFLIN

分子量

Approximately 21 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a solution containing 1X PBS, pH 7.4, trehalose.

内毒素含量

<0.1 EU per 1 μg of the protein by the LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Animal-Free TRAIL/TNFSF10 Protein, Mouse (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Animal-Free TRAIL/TNFSF10 Protein, Mouse (His)
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HY-P7089AF
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