ANO1/Anoctamin-1 蛋白, Human (N-His, C-Myc)

ANO1/Anoctamin-1 protein, a calcium-activated chloride channel (CaCC), is involved in ion transport, smooth muscle contraction, and mucus secretion. ANO1/Anoctamin-1 Protein, Human (N-His, C-Myc) is the recombinant human-derived ANO1/Anoctamin-1 protein, expressed by E. coli , with C-Myc, N-10*His labeled tag.

ANO1/Anoctamin-1 蛋白是一种钙激活氯离子通道 (CaCC)，在细胞功能中发挥多种作用。它是气道上皮细胞中基础氯电导和刺激氯电导的主要贡献者，影响气管软骨发育并促进白细胞介素 IL3 和 IL8 以及哮喘相关蛋白 CLCA1 诱导的气道粘液表达。ANO1 对于 Cajal 间质细胞 (ICC) 的正常功能是必不可少的，对于胃肠道平滑肌中的电起搏器活动至关重要，并且参与跨上皮阴离子转运和平滑肌收缩。CFTR 激活和膜表达、调节内质网 Ca(2+) 储存释放也需要它。此外，ANO1 参与人汗腺上皮细胞中钙激活的氯离子分泌，显示基础氯离子渗透性增加和 Ca(2+) 诱导的氯离子渗透性降低。在感觉神经元中，ANO1 充当热传感器并调节自发放电模式，促进味觉细胞中钙激活的氯离子通道活性，并介导背根神经节中非组胺能 Mas 相关 G 蛋白偶联受体 (MRGPR) 依赖性瘙痒神经元。在发育中的大脑中，ANO1 对于放射状胶质细胞的 Ca(2+) 依赖性过程延伸至关重要。

Human

E. coli

C-Myc;N-10*His

Q5XXA6-1 (S806-A892)

55107  [NCBI]

SFTSDFIPRLVYLYMYSKNGTMHGFVNHTLSSFNVSDFQNGTAPNDPLDLGYEVQICRYKDYREPPWSENKYDISKDFWAVLAARLA

Approximately 17.6 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.