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  3. Annexin A4/ANXA4 蛋白, Mouse (His-Myc)

Annexin A4/ANXA4 蛋白, Mouse (His-Myc)

目录号: HY-P72079
COA 产品使用指南

膜联蛋白 A4/ANXA4 蛋白是一种钙/磷脂结合蛋白,通过促进膜融合和胞吐作用在膜动力学中发挥关键作用。这种蛋白质对钙离子和磷脂表现出特定的亲和力,使其能够参与涉及膜相互作用的细胞过程。Annexin A4/ANXA4 蛋白, Mouse (His-Myc) 是重组的 膜联蛋白 A4/ANXA4 蛋白,由 E. coli 表达,带有 N-10*His, C-Myc 标签。Annexin A4/ANXA4 蛋白, Mouse (His-Myc) 全长 318 个氨基酸,分子量约为 ~40.8 kDa。

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描述

The Annexin A4/ANXA4 protein is a calcium/phospholipid-binding protein that plays a key role in membrane dynamics by promoting membrane fusion and exocytosis. This protein exhibits specific affinity for calcium ions and phospholipids, allowing it to participate in cellular processes involving membrane interactions. Annexin A4/ANXA4 Protein, Mouse (His-Myc) is the recombinant mouse-derived Annexin A4/ANXA4 protein, expressed by E. coli , with N-10*His, C-Myc labeled tag. The total length of Annexin A4/ANXA4 Protein, Mouse (His-Myc) is 318 a.a., with molecular weight of ~40.8 kDa.

研究背景

膜联蛋白 A4 (ANXA4) 是一种钙/磷脂结合蛋白,以其在膜融合和参与胞吐作用中的关键作用而闻名。ANXA4 作为钙信号传导和膜动力学之间的介质,有助于参与细胞内容物释放的细胞过程。它与钙离子和磷脂结合的能力表明在膜相关事件中具有调节作用,特别是与囊泡运输和胞吐作用相关的事件。它必须强调 ANXA4 在钙依赖性细胞机制中的重要性,强调其参与促进膜融合和促进胞吐过程。

种属

Mouse

表达系统

E. coli

标签

N-10*His;C-Myc

蛋白编号

P97429 (E2-D319)

基因 ID
蛋白结构
N-term
10*His
ANXA4 (E2-D319)
Accession # P97429
Myc
C-term
同用名
Anxa4; Anx4Annexin A4; Annexin IV; Annexin-4
氨基酸序列

EAKGGTVKAASGFNATEDAQTLRKAMKGLGTDEDAIIGILAYRNTAQRQEIRSAYKSTIGRDLIEDLKSELSSNFEQVILGLMTPTVLYDVQELRRAMKGAGTDEGCLIEILASRTPEEIRRINQTYQQQYGRSLEEDICSDTSFMFQRVLVSLSAAGRDEGNYLDDALMKQDAQELYEAGEKRWGTDEVKFLSILCSRNRNHLLHVFDEYKRISQKDIEQSIKSETSGSFEDALLAIVKCMRSKPSYFAERLYKSMKGLGTDDNTLIRVMVSRAEIDMLDIRASFKRLYGKSLYSFIKGDTSGDYRKVLLVLCGGDD

分子量

Approximately 40.8 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm solution of 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料

Annexin A4/ANXA4 Protein, Mouse (His-Myc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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