Apolipoprotein A-II/APOA2 蛋白, Human (HEK293, His)

Apolipoprotein A-II/ApoA2 Protein, Human (HEK293, His) expresses in HEK293 with a His tag at the N-terminus. Apolipoprotein A-II (ApoA-II) has been associated with numerous aspects of HDL metabolism, and apoA-II overexpression produces the proatherogenic phenotype^[1].

重组 apoA-II 在结合和重组脂质以及通过 ATP 结合盒转运蛋白 A1 促进胆固醇从巨噬细胞流出的能力方面与血浆形式相当。apoA-II 主要在肝脏中合成，是一种由 77 个氨基酸组成的蛋白质，其位置上有一个半胱氨酸。在健康人体中，apoA-II 几乎完全以二硫键连接的同二聚体形式存在（分子量为 17.4 kDa），但偶尔也会发现它与其他含 Cys 的蛋白质形成异二聚体^[1]。

Human

HEK293

C-6*His

P02652 (Q24-Q100)

336  [NCBI]

QAKEPCVESLVSQYFQTVTDYGKDLMEKVKSPELQAEAKSYFEKSKEQLTPLIKKAGTELVNFLSYFVELGTQPATQHHHHHH

10-15 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Smith LE, et al. High yield expression and purification of recombinant human apolipoprotein A-II in Escherichia coli. J Lipid Res. 2012 Aug;53(8):1708-15.  [Content Brief]