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  3. Apolipoprotein B-100/APOB 蛋白, Human (His, solution)

Apolipoprotein B-100/APOB 蛋白, Human (His, solution)

目录号: HY-P7524
COA 产品使用指南

Apolipoprotein B-100/Apo B 蛋白, Human (His, solution) 在 E. coli 中表达,N 端带有一个 His 标签。是低密度脂蛋白的主要载脂蛋白,并且是与低密度脂蛋白受体相互作用的主要配体。

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规格 价格 是否有货 数量
10 μg ¥2100 In-stock
50 μg ¥5900 询价
100 μg   询价  

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描述

Apolipoprotein B-100/Apo B Protein, Human (His, solution) expresses in E. coli with a His tag at the N-terminus. Human Apolipoprotein B-100 (apo B-100) is the major apolipoprotein of low density lipoproteins and the principal ligand for interaction with the low density lipoprotein receptor[1].

研究背景

人apo B-100的克隆为apo B-100的结构和理化特性提供了新的见解,并将有助于研究调节apo B-100生物合成的因素以及异常脂蛋白血症患者中apo B-100基因的表达[1]

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

P04114 (E28-S127)

基因 ID

338  [NCBI]

蛋白结构
N-term
6*His
APOB (E28-S127)
Accession # P04114
C-term
同用名
rHuApolipoproteinB-100, His; APOB; ApolipoproteinB-100
氨基酸序列

HHHHHHEEEMLENVSLVCPKDATRFKHLRKYTYNYEAESSSGVPGTADSRSATRINCKVELEVPQLCSFILKTSQCTLKEVYGFNPEGKALLKKTKNSEEFAAAMS

分子量

Approximately 16.0 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filter solution of 20 mM Tris-HCl, 250 mM NaCl, 10% Glycerol, 2 mM EDTA, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料
参考文献

Apolipoprotein B-100/APOB Protein, Human (His, solution) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Apolipoprotein B-100/APOB Protein, Human (His, solution)
目录号:
HY-P7524
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