Apolipoprotein E/APOE2 蛋白, Human (R154S, R176C, HEK293, C-His)

Apolipoprotein E (APOE) plays a key role in lipoprotein-mediated lipid transport and is a core component in the production, transformation, and clearance of plasma lipoproteins. As an amphipathic molecule, APOE binds to various lipoprotein particles, including chylomicrons, chylomicron remnants, VLDL, and IDL, favoring HDL. Apolipoprotein E/APOE Protein, Human (R154S, R176C, HEK293, C-His) is the recombinant human-derived Apolipoprotein E/APOE protein, expressed by HEK293 , with C-6*His labeled tag and R154S, R176C, , , mutation. The total length of Apolipoprotein E/APOE Protein, Human (R154S, R176C, HEK293, C-His) is 299 a.a., with molecular weight of 33-42 kDa.

载脂蛋白 E (APOE) 在脂蛋白介导的脂质转运中发挥着重要作用，是血浆脂蛋白的核心成分，参与其产生、转化和清除。作为两亲性分子，APOE 与各种脂蛋白颗粒结合，包括乳糜微粒、乳糜微粒残余物、极低密度脂蛋白 (VLDL) 和中密度脂蛋白 (IDL)，并优先结合高密度脂蛋白 (HDL)。它与一系列细胞受体结合，例如 LDL 受体 (LDLR)、LDL 受体相关蛋白（LRP1、LRP2 和 LRP8）以及极低密度脂蛋白受体 (VLDLR)，促进细胞摄取 APOE-含有脂蛋白颗粒。此外，APOE 具有肝素结合活性，与细胞表面的硫酸乙酰肝素蛋白聚糖相互作用，支持捕获和受体介导的含 APOE 脂蛋白的摄取。APOE的主要功能包括通过肝细胞摄取介导脂蛋白清除，并参与外周组织VLDL的生物合成和摄取以进行甘油三酯输送和能量储存。它对脂质体内平衡至关重要，参与胆固醇反向转运，并在中枢神经系统、免疫反应和转录调节中发挥作用，特别是在微生物感染期间与 HCV 的相互作用中。

Measured in a cell proliferation assay using SH-SY5Y Human neuroblastoma cells. The ED₅₀ this effect is ≤8.753 ng/mL. corresponding to a specific activity is ≥1.14×10⁵ units/mg.

Human

HEK293

C-6*His

P02649 (K19-H317, R154S, R176C)

348  [NCBI]

KVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVSLASHLRKLRKRLLRDADDLQKCLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH

Approximately 33-42 kDa due to the glycosylation

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized a 0.22 μm filtered solution of PBS, 2 mM CHAPS, 2 mM DTT, 1 mM PMSF, pH 7.4 (Normally trehalose is added as protectant before lyophilization ) or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.