Apolipoprotein M/APOM 蛋白, Human (HEK293, His)

Apolipoprotein M Protein, Human (HEK293, His) expresses in HEK293 with a His tag at the N-terminus. Apolipoprotein E (apoE) secreted by HEK cells stably expressing apoE3 or apoE4 (HEK-apoE) binds Aβ, and inhibits Aβ-induced neurotoxicity ^[1]. The signal peptide is not cleaved and is required for ApoM association with lipoprotein particles as well as Megalin mediated reabsorption by the kidney. ApoM is cleared from the circulation by the ubiquitously expressed LDL R.

载脂蛋白 E (apoE) 是一种 34 kDa 糖蛋白，是各种血浆脂蛋白颗粒的表面成分，包括乳糜微粒残留物、β-迁移 VLDL (β-VLDL)、LDL 和 HDL 亚类。在血浆胆固醇 (Chol) 和甘油三酯 (TG) 的运输和代谢过程中，ApoE 介导含 apoE 的脂蛋白与细胞表面内吞受体的高亲和力结合。HEK-apoE 是低密度脂蛋白 (LDL) 受体相关蛋白 (LRP) 的配体，但不是 LDL 受体^[1]。

Human

HEK293

C-6*His

O95445-1 (M1-N188)

55937  [NCBI]

CPEHSQLTTLGVDGKEFPEVHLGQWYFIAGAAPTKEELATFDPVDNIVFNMAAGSAPMQLHLRATIRMKDGLCVPRKWIYHLTEGSTDLRTEGRPDMKTELFSSSCPGGIMLNETGQGYQRFLLYNRSPHPPEKCVEEFKSLTSCLDSKAFLLTPRNQEACELSNN

19-24 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized after extensive dialysis against PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. LaDu MJ, et al. Self-assembly of HEK cell-secreted ApoE particles resembles ApoE enrichment of lipoproteins as a ligand for the LDL receptor-related protein. Biochemistry. 2006 Jan 17;45(2):381-90.  [Content Brief]