ARF1 蛋白, Human (His-GST, Myc)

ARF1 is a small GTPase responsible for coordinating protein transport within the Golgi complex. In its GTP-bound state, ARF1 critically regulates Golgi vesicle budding and uncoating by recruiting coat proteins. ARF1 Protein, Human (His-GST, Myc) is the recombinant human-derived ARF1 protein, expressed by E. coli , with N-10*His, C-Myc, GST labeled tag. The total length of ARF1 Protein, Human (His-GST, Myc) is 180 a.a., with molecular weight of ~55 kDa.

ARF1 是一种小型 GTP 酶，复杂地参与不同细胞区室之间的蛋白质运输，特别是在高尔基复合体内。在其 GTP 结合状态下，ARF1 在调节高尔基体内囊泡出芽和脱壳过程中发挥着关键作用，协调外壳蛋白向高尔基体膜的募集。随后由 ARFGAP 蛋白促进的 ARF1 结合 GTP 的水解对于外壳蛋白从高尔基体膜和囊泡的解离至关重要。此外，GTP 结合形式的 ARF1 与 PICK1 相互作用，调节 AMPA 受体 (AMPAR) 运输并影响兴奋性突触的突触可塑性，以及长期抑郁 (LTD) 期间的脊柱收缩。在微生物感染的情况下，ARF1 作为霍乱毒素催化亚基的变构激活剂发挥着意想不到的作用，在 ADP-核糖基转移酶活性中发挥作用。

Human

E. coli

N-10*His;C-Myc;GST

P84077 (G2-K181)

375  [NCBI]

GNIFANLFKGLFGKKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK

Approximately 55 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.