ARMET/MANF 蛋白, Human (His)

ARMET/MANF proteins critically support the survival of dopaminergic neurons in the ventral midbrain and contribute to neuronal homeostasis. It modulates GABAergic transmission and enhances inhibitory postsynaptic currents in substantia nigra dopaminergic neurons. ARMET/MANF Protein, Human (His) is the recombinant human-derived ARMET/MANF protein, expressed by E. coli , with N-6*His labeled tag. The total length of ARMET/MANF Protein, Human (His) is 158 a.a., with molecular weight of ~20 kDa.

ARMET/MANF 蛋白选择性促进中脑腹侧多巴胺能神经元的存活，从而在神经元稳态中发挥至关重要的作用。它调节 GABA 能向黑质中多巴胺能神经元的传递，增强自发和诱发的 GABA 能抑制性突触后电流，表明其参与神经传递调节（通过相似性）。此外，ARMET/MANF 抑制细胞增殖并防止内质网 (ER) 应激诱导的细胞死亡，证明其在细胞过程中的多方面作用。在正常情况下，该蛋白通过与内质网伴侣蛋白 HSPA5 结合而保留在 ER/肌浆网 (SR) 中。然而，为了应对 ER 应激和缺氧，ARMET/MANF 被 ER/SR 上调和分泌，导致其与 3-O-磺基半乳糖神经酰胺（靶细胞外细胞膜中的一种脂硫脂）结合。这种相互作用通过内吞作用促进细胞摄取，对于 ARMET/MANF 在缺氧和 ER 应激条件下减轻 ER 应激和减轻细胞毒性的作用至关重要。该蛋白还直接与 HSPA5 相互作用，并且是涉及 CRELD2、MANF、MATN3 和 PDIA4 的复合物的一部分，进一步强调了其参与细胞调节网络（通过相似性）。

Measured in a cell proliferation assay using rat C6 cells.The ED₅₀ for this effect is ≤0.8862 μg/mL, corresponding to a specific activity is ≥1.13×10³ units/mg.

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  Measured in a cell proliferation assay using rat C6 cells.The ED₅₀ for this effect is 0.8862 μg/mL, corresponding to a specific activity is 1.13×10³ units/mg.

Human

E. coli

N-6*His

P55145 (L25-L182)

7873  [NCBI]

LRPGDCEVCISYLGRFYQDLKDRDVTFSPATIENELIKFCREARGKENRLCYYIGATDDAATKIINEVSKPLAHHIPVEKICEKLKKKDSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGETCKGCAEKSDYIRKINELMPKYAPKAASARTDL

Approximately 20 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.