ASGR1/ASGPR1 蛋白, Mouse (HEK293, His)

ASGR1/ASGPR1 proteins play a crucial role in cellular processes by mediating endocytosis of desialylated plasma glycoproteins and recognizing terminal galactose and N-acetylgalactosamine. It promotes ligand internalization and formation of complexes that are transported to sorting organelles. ASGR1/ASGPR1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ASGR1/ASGPR1 protein, expressed by HEK293 , with N-His labeled tag. The total length of ASGR1/ASGPR1 Protein, Mouse (HEK293, His) is 225 a.a., with molecular weight of 30-45 kDa.

ASGR1/ASGPR1 蛋白通过介导血浆糖蛋白的内吞作用在细胞过程中发挥至关重要的作用，血浆糖蛋白在复杂碳水化合物部分上的末端唾液酸残基已被去除。受体对末端半乳糖和 N-乙酰半乳糖胺单元的识别使配体内化，形成复合物，随后被转运至分选细胞器。在该细胞器内，受体和配体解离，ASGR1/ASGPR1 被循环回到细胞膜表面。该蛋白质参与这些动态过程强调了其在糖蛋白的细胞处理中的重要性，并有助于细胞稳态的调节。值得注意的是，ASGR1/ASGPR1 还与 LASS2 相互作用，扩大了其分子关联，并表明其对细胞信号传导或协调的潜在影响。

Measured by its binding ability in a functional ELISA. Immobilized ASGR1 at 2.5 μg/mL (100 μL/well) can bind Biotinylated Von Willebrand Factor. The ED₅₀ for this effect is 0.8278 μg/mL, corresponding to a specific activity is 1.21×10³ Unit/mg.

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  Measured by its binding ability in a functional ELISA. Immobilized ASGR1 at 2.5 μg/mL (100 μL/well) can bind Biotinylated Von Willebrand Factor. The ED₅₀ for this effect is 0.8278 μg/mL, corresponding to a specific activity is 1.21×10³ Unit/mg.

Mouse

HEK293

N-His

P34927 (S60-N284)

11889  [NCBI]

SQNSQLREDLLALRQNFSNLTVSTEDQVKALSTQGSSVGRKMKLVESKLEKQQKDLTEDHSSLLLHVKQLVSDVRSLSCQMAAFRGNGSERTCCPINWVEYEGSCYWFSSSVRPWTEADKYCQLENAHLVVVTSRDEQNFLQRHMGPLNTWIGLTDQNGPWKWVDGTDYETGFQNWRPEQPDNWYGHGLGGGEDCAHFTTDGRWNDDVCRRPYRWVCETKLDKAN

30-45 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.