ASK1 蛋白, Human (His)

ASK1 protein is a key serine/threonine kinase in the MAP kinase pathway, coordinating cellular responses to environmental changes and affecting cell fate, differentiation, and survival. ASK1 is a key player in apoptosis and promotes mitochondria-dependent caspase activation. ASK1 Protein, Human (His) is the recombinant human-derived ASK1 protein, expressed by E. coli , with C-6*His labeled tag. The total length of ASK1 Protein, Human (His) is 298 a.a., .

ASK1 是一种丝氨酸/苏氨酸激酶，作为 MAP 激酶信号转导途径的关键组成部分，在协调细胞对环境变化的反应中发挥着至关重要的作用。该激酶有助于确定细胞命运，影响分化和存活等过程。值得注意的是，ASK1 是细胞凋亡信号转导途径的关键参与者，促进线粒体依赖性 caspase 激活。此外，MAP3K5/ASK1 对于先天免疫反应是不可或缺的，对宿主防御多种病原体有显着贡献。它作为响应各种应激源（包括氧化应激）的信号转导介质，并对受体介导的炎症信号做出响应，例如由肿瘤坏死因子（TNF）或脂多糖（LPS）引发的炎症信号。激活后，ASK1 通过磷酸化和激活 MAP 激酶激酶（如 MAP2K4/SEK1、MAP2K3/MKK3、MAP2K6/MKK6 和 MAP2K7/MKK7）充当 MKK/JNK 和 p38 MAPK 信号转导级联的上游激活剂。这些激活的 MAP2K 反过来启动 p38 MAPK 和 c-jun N 末端激酶 (JNK) 的激活，这两者都对激活蛋白 1 (AP-1) 家族的转录因子进行控制。

Human

E. coli

C-6*His

Q99683 (M649-K946)

4217

Approximately 34.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.