Fibrillin-1/Asprosin 蛋白, Human (HEK293, His)

Fibrillin-1 and Asprosin are two proteins generated from the same precursor protein, which is encoded by the Fibrillin 1 gene, through proteolytic cleavage. Fibrillin-1 protein, a member of the fibrillin family, is a crucial structural protein in the formation of microfibrils in the extracellular matrix, maintaining tissue homeostasis and cell adhesion, regulating the availability of growth factors, and inhibiting osteoclastogenesis. In contrast, Asprosin is a hormone primarily secreted by white adipose tissue and is involved in regulating glucose metabolism within the body. Fibrillin-1/Asprosin protein, Human (HEK293, His) is a recombinant protein expressed in HEK293 cells with an N-terminal His tag, comprising a full length of 140 amino acids (S2732-H2871)^{[1][2][3][4][5][6][7][8][9][10][11]}.

Fibrillin-1 是一种细胞外基质微纤维形成的关键结构蛋白。Fibrillin-1 对结缔组织的结构完整性和功能稳定性具有重要作用，不仅提供了物理支持，还通过与多种生长因子、整合素和肝素相互作用，参与组织的稳态调节和骨骼代谢调控。
1. 物理结构支持：
(1) 在肺、血管和皮肤等组织中，Fibrillin-1 支持弹性纤维的形成^[1]。
(2) 在没有弹性蛋白的组织中，包括睫状韧带、肌腱、角膜和肾小球等组织中，提供结构支持^[1]。
2. 稳态调节与代谢调控：
(1) Fibrillin-1 通过与生长因子 (如骨形态发生蛋白 (BMPs)、增长和分化因子 (GDFs)、转化生长因子 β 结合蛋白 (LTBPs))、细胞表面整合素及其他细胞外基质蛋白和蛋白聚糖成分的特异性相互作用，维持组织结构的稳定性^[1]。
(2) Fibrillin-1 通过结合破骨细胞分化因子 TNFSF11，抑制破骨细胞的生成，并抑制 TNFSF11 介导的转录因子 NFATC1 的核转位和激活，进而影响骨代谢^[2]。
Fibrillin-1 通过与细胞表面受体整合素 ITGAV 和 ITGA5 结合，介导细胞黏附^[3][4]。它还可以与肝素结合，确保微纤维的正确组装^[5]。
Asprosin 是一种由白色脂肪组织分泌的激素，在调节体内能量平衡以及保护心脏方面发挥重要作用。Asprosin 在禁食状态下作用于肝脏，通过与肝细胞上的嗅觉受体 OR4M1 结合，激活蛋白激酶 A，从而引发肝细胞快速释放葡萄糖进入血液循环。同时 Asprosin 也可以通过激活 G 蛋白-cAMP-PKA 途径促使葡萄糖快速释放^[6][7][8][9]。

Fibrillin-1 protein (Human) (0-20 μg/ mL，48 h) 剂量依赖性地促进 αvβ3 和 α5β1 整合素介导的人脐静脉内皮细胞的黏附，并在 1 μg/mL 的剂量下加强其增殖和迁移^[10]。

Asprosin protein (Human) (2 mg/kg，腹腔注射，单次给药，在给药后 48 小时或 7 天抽取血样) Isoprenaline hydrochloride (HY-B0468) 诱导的心肌梗死大鼠模型中具有心脏保护作用^[11].

1.Measured by its binding ability in a functional ELISA. Immobilized Human MFAP4 at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human Fibrillin-1. The ED₅₀ for this effect is ≤0.8473 μg/mL, corresponding to a specific activity is ≥1.18×10³ Unit/mg.
2.Measured by its ability to induce TNF-ɑ secretion by macrophages produced by THP-1 cells treated with dihydroxyvitamin D3.

• 
  Measured by its binding ability in a functional ELISA. Immobilized Human MFAP4 at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human Fibrillin-1. The ED₅₀ for this effect is 0.6163 μg/mL, corresponding to a specific activity is 1.62×10³ Unit/mg.

Human

HEK293

N-8*His

P35555 (S2732-H2871)

2200  [NCBI]

STNETDASNIEDQSETEANVSLASWDVEKTAIFAFNISHVSNKVRILELLPALTTLTNHNRYLIESGNEDGFFKINQKEGISYLHFTKKKPVAGTYSLQISSTPLYKKKELNQLEDKYDKDYLSGELGDNLKMKIQVLLH

Approximately 25-33 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Jensen SA, et al. New insights into the structure, assembly and biological roles of 10-12 nm connective tissue microfibrils from fibrillin-1 studies. Biochem J. 2016 Apr 1;473(7):827-38.  [Content Brief]

  [2]. Tiedemann K, et al. Fibrillin-1 directly regulates osteoclast formation and function by a dual mechanism. J Cell Sci. 2013 Sep 15;126(Pt 18):4187-94.  [Content Brief]

  [3]. Bax DV, et al. Cell adhesion to fibrillin-1 molecules and microfibrils is mediated by alpha 5 beta 1 and alpha v beta 3 integrins. J Biol Chem. 2003 Sep 5;278(36):34605-16.  [Content Brief]

  [4]. Jovanovic J, et al. alphaVbeta6 is a novel receptor for human fibrillin-1. Comparative studies of molecular determinants underlying integrin-rgd affinity and specificity. J Biol Chem. 2007 Mar 2;282(9):6743-51.  [Content Brief]

  [5]. Tiedemann K, et al. Interactions of fibrillin-1 with heparin/heparan sulfate, implications for microfibrillar assembly. J Biol Chem. 2001 Sep 21;276(38):36035-42.  [Content Brief]

  [6]. Romere C, et al. Asprosin, a Fasting-Induced Glucogenic Protein Hormone. Cell. 2016 Apr 21;165(3):566-79.  [Content Brief]

  [7]. Lee T, et al. Asprosin impairs insulin secretion in response to glucose and viability through TLR4/JNK-mediated inflammation. Mol Cell Endocrinol. 2019 Apr 15;486:96-104.  [Content Brief]

  [8]. Li E, et al. OLFR734 Mediates Glucose Metabolism as a Receptor of Asprosin. Cell Metab. 2019 Aug 6;30(2):319-328.e8.  [Content Brief]

  [9]. Li E, et al. OLFR734 Mediates Glucose Metabolism as a Receptor of Asprosin. Cell Metab. 2019 Aug 6;30(2):319-328.e8.  [Content Brief]

  [10]. Mariko B, et al. Microfibrils and fibrillin-1 induce integrin-mediated signaling, proliferation and migration in human endothelial cells. Am J Physiol Cell Physiol. 2010 Nov;299(5):C977-87.  [Content Brief]

  [11]. Onat E, et al. The protective effects of humanin in rats with experimental myocardial infarction: The role of asprosin and spexin. Heliyon. 2023 Jul 26;9(8):e18739.  [Content Brief]