ASXL1 蛋白, Human (GST)

ASXL1 Protein, Human (GST) is a recombinant human Additional Sex Combs-like Protein 1 (ASXL1) expressed in E. coli with a GST tag. ASXLs make biological and functional roles in development, cancer, and transcription^[1].

附加性梳状 (ASXL) 蛋白是附加性梳 (Asx) 的哺乳动物同源物，后者是调节果蝇中三胸和多梳功能平衡的蛋白质。所有三个 ASXL 家族成员（ASXL1、ASXL2 和 ASXL3）分别受到癌症或罕见发育综合征中的体细胞突变或从头种系突变的影响^[1]。

Human

E. coli

N-GST

Q8IXJ9-1 (K1477-R1541)

171023  [NCBI]

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKKANFGASHSASLSLQMFTDSSTVESISLQCACSLKAMIMCQGCGAFCHDDCIGPSKLCVLCLVVR

Approximately 38.0 kDa

Greater than 82% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Jean-Baptiste Micol, et al. The Role of Additional Sex Combs-Like Proteins in Cancer. Cold Spring Harb Perspect Med. 2016 Oct 3;6(10):a026526.  [Content Brief]