ATF1 蛋白, Human (His)

ATF1 Protein, Human (His) expressed in E. coli with a His tag. ATF1 is a member of the ATF/CREB family of transcription factors (TFs), specifically interacting with the consensus ATF/CRE site ‘TGACGTCA’^[1].

激活转录因子 1 (ATF1) 是 ATF/CREB 转录因子 (TF) 家族的成员，专门与 ATF/CRE 共有位点“TGACGTCA”相互作用。CREB 和 ATF1 是 t-Darpp 上调 Bcl-2 水平以抵抗神经酰胺诱导的胃癌细胞凋亡所必需的。此外，在黑色素瘤细胞中表达抗 ATF1 单链抗体片段可抑制裸鼠中 ATF1 的致瘤性和转移潜力^[1]。

Human

E. coli

C-6*His

P18846 (M1-V271)

466  [NCBI]

MEDSHKSTTSETAPQPGSAVQGAHISHIAQQVSSLSESEESQDSSDSIGSSQKAHGILARRPSYRKILKDLSSEDTRGRKGDGENSGVSAAVTSMSVPTPIYQTSSGQYIAIAPNGALQLASPGTDGVQGLQTLTMTNSGSTQQGTTILQYAQTSDGQQILVPSNQVVVQTASGDMQTYQIRTTPSATSLPQTVVMTSPVTLTSQTTKTDDPQLKREIRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKTLKDLYSNKSVHHHHHH

Approximately 34.8 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Guo-Liang Huang, et al. The protein level and transcription activity of activating transcription factor 1 is regulated by prolyl isomerase Pin1 in nasopharyngeal carcinoma progression. Cell Death Dis. 2016 Dec 29;7(12):e2571.  [Content Brief]