ATP6AP2 蛋白, Human (His-SUMO)

The multifunctional ATP6AP2 protein serves as a cellular receptor for renin and prorenin, contributing to lysosomal V-ATPase assembly and endolysosomal acidification. It participates in renin-dependent reactions, activates ERK1/2, and may enhance the catalytic efficiency of renin in the renin-angiotensin system. ATP6AP2 Protein, Human (His-SUMO) is the recombinant human-derived ATP6AP2 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of ATP6AP2 Protein, Human (His-SUMO) is 334 a.a., with molecular weight of ~53.5 kDa.

ATP6AP2 蛋白是细胞过程中的多功能参与者，充当肾素和肾素原细胞受体，同时有助于溶酶体质子转运 V 型 ATP 酶 (V-ATP 酶) 的组装和内溶酶体系统的酸化。它参与肾素依赖性细胞反应，激活 ERK1 和 ERK2，并通过增强肾素在 AGT/血管紧张素原转化为血管紧张素 I 的催化效率，在肾素-血管紧张素系统 (RAS) 中发挥潜在作用。ATP 酶组装和溶酶体酸化调节蛋白质降解，影响对大脑正常发育、突触形态和突触传递至关重要的信号通路。ATP6AP2 与肾素相互作用，并作为 V-ATP 酶蛋白泵的辅助组件发挥作用。它与 ATP6AP1、ATP6V0D1、TMEM9 和 VMA21 的相互作用进一步强调了它在 V-ATP 酶复合物的组装和调节中的复杂作用。这种多方面的功能使 ATP6AP2 成为各种细胞通路中的关键协调器。

Human

E. coli

N-6*His;N-SUMO

O75787 (N17-D350)

10159  [NCBI]

NEFSILKSPGSVVFRNGNWPIPGERIPDVAALSMGFSVKEDLSWPGLAVGNLFHRPRATVMVMVKGVNKLALPPGSVISYPLENAVPFSLDSVANSIHSLFSEETPVVLQLAPSEERVYMVGKANSVFEDLSVTLRQLRNRLFQENSVLSSLPLNSLSRNNEVDLLFLSELQVLHDISSLLSRHKHLAKDHSPDLYSLELAGLDEIGKRYGEDSEQFRDASKILVDALQKFADDMYSLYGGNAVVELVTVKSFDTSLIRKTRTILEAKQAKNPASPYNLAYKYNFEYSVVFNMVLWIMIALALAVIITSYNIWNMDPGYDSIIYRMTNQKIRMD

Approximately 53.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.