1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. ATXN3L 蛋白, Human (Sf9, His, FLAG)

ATXN3L 蛋白, Human (Sf9, His, FLAG)

目录号: HY-P701461
产品使用指南

ATXN3L 蛋白是一种去泛素化酶,通过在体外裂解“Lys-48”连接和“Lys-63”连接的多聚泛素链而表现出多种活性。它作为转录因子 KLF5 的特异性去泛素酶发挥作用,有助于 KLF5 的稳定性调节。ATXN3L 蛋白, Human (Sf9, His, FLAG) 是重组的 ATXN3L 蛋白,由 Sf9 insect cells 表达,带有 C-Flag, N-8*His 标签。ATXN3L 蛋白, Human (Sf9, His, FLAG) 全长 354 个氨基酸。

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描述

The ATXN3L protein is a deubiquitinating enzyme that exhibits multiple activities by cleaving “Lys-48”-linked and “Lys-63”-linked polyubiquitin chains in vitro. It functions as a specific deubiquitinase for the transcription factor KLF5 and contributes to the stability regulation of KLF5. ATXN3L Protein, Human (Sf9, His, FLAG) is the recombinant human-derived ATXN3L protein, expressed by Sf9 insect cells , with C-Flag, N-8*His labeled tag. The total length of ATXN3L Protein, Human (Sf9, His, FLAG) is 354 a.a., .

研究背景

ATXN3L 蛋白作为一种去泛素化酶,通过在体外裂解“Lys-48”连接和“Lys-63”连接的多聚泛素链而表现出多种活性。此外,它作为转录因子 KLF5 的特异性去泛素酶,从而有助于 KLF5 稳定性的调节。ATXN3L 调节多种泛素连接并与特定转录因子相互作用的双重能力强调了其在细胞过程和蛋白质稳定性动态调节中的作用,揭示了其在细胞稳态的复杂景观中的重要性。

种属

Human

表达系统

Sf9 insect cells

标签

C-Flag;N-8*His

蛋白编号

Q9H3M9 (D2-K355)

基因 ID

92552

蛋白结构
N-term
8*His
ATXN3L (D2-K355)
Accession # Q9H3M9
Flag
C-term
同用名
ATXN3L; Ataxin-3-like protein; Machado-Joseph disease protein 1-like
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ATXN3L Protein, Human (Sf9, His, FLAG) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ATXN3L Protein, Human (Sf9, His, FLAG)
目录号:
HY-P701461
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