Azoreductase/NQO1 蛋白, Human (His)

Azo reductase/NQO1 protein is a flavin-containing quinone reductase that uses NADH or NADPH to catalyze the two-electron reduction of quinone to hydroquinone. It regulates cellular redox status by detoxifying quinones and reducing plasma membrane redox components. Azoreductase/NQO1 Protein, Human (His) is the recombinant human-derived Azoreductase/NQO1 protein, expressed by E. coli , with N-His labeled tag. The total length of Azoreductase/NQO1 Protein, Human (His) is 274 a.a., with molecular weight of ~33 kDa.

偶氮还原酶/NQO1 蛋白是一种含黄素的醌还原酶，利用 NADH 或 NADPH 作为电子供体，促进醌双电子还原为氢醌。通过乒乓动力学机制，电子依次从 NAD(P)H 转移到黄素辅因子，然后转移到醌，有效地绕过了半醌和活性氧的生成。这种酶活性在通过解毒醌来调节细胞氧化还原平衡方面发挥着至关重要的作用。偶氮还原酶/NQO1 作为超氧化物清除剂，防止对苯二酚氧化并支持抗氧化防御机制。此外，它还参与醌的激活，通过 DNA 交联生成具有潜在抗肿瘤特性的氧化还原反应性氢醌。值得注意的是，该蛋白充当核心 20S 蛋白酶体的看门人，以 NADH 依赖性方式与肿瘤抑制因子 TP53 和 TP73 相互作用，以抑制氧化应激期间其不依赖于泛素的降解。

Biological activity has been tested in vitro.

Human

E. coli

N-His

P15559-1 (M1-K274)

1728  [NCBI]

MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKKRLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK

Approximately 33 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.