CD276/B7-H3 蛋白, Human (435a.a, HEK293, Fc)

CD276/B7-H3 Protein, Human (HEK293, Fc) is a polypeptide chain containing the C-termimal His tag produced in HEK293 cells. B7-H3 is an immune checkpoint from the B7 family of molecules.

作为B7家族的一员，肿瘤细胞上表达的诱导型共刺激配体（ICOSLG）已被报道在肿瘤免疫中发挥重要作用。作为 CD28 相关分子 ICOS 的反配体，ICOLSG 在专职抗原呈递细胞（APC；如树突状细胞 (DC) 和 B 细胞）和某些肿瘤细胞（如神经胶质瘤细胞和胃癌细胞）上表达）。ICOSLG 是唯一优先共同刺激 2T 型辅助细胞 (Th2) 反应的 B7 家族成员，有趣的是，这种独特的分子在抗肿瘤免疫中具有关键作用。实体瘤（植入转染肿瘤）上的 ICOSLG 表达有助于 NK 介导的和 CD8⁺ 细胞毒性 T 细胞 (CTL) 的激活和杀伤。使用 ICOSLG 转染的实体瘤细胞系进行的多项研究发现，ICOLSG 诱导 CD8⁺ 细胞毒性淋巴细胞介导的肿瘤消退^[1]。

Human

HEK293

C-hFc

Q5ZPR3-1 (L29-P463)

80381  [NCBI]

LEVQVPEDPVVALVGTDATLCCSFSPEPGFSLAQLNLIWQLTDTKQLVHSFAEGQDQGSAYANRTALFPDLLAQGNASLRLQRVRVADEGSFTCFVSIRDFGSAAVSLQVAAPYSKPSMTLEPNKDLRPGDTVTITCSSYRGYPEAEVFWQDGQGVPLTGNVTTSQMANEQGLFDVHSVLRVVLGANGTYSCLVRNPVLQQDAHGSVTITGQPMTFP

62-65 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Wang B, et al. Effects of ICOSLG expressed in mouse hematological neoplasm cell lines in the GVL reaction. Bone Marrow Transplant. 2013 Jan;48(1):124-8.  [Content Brief]