Basigin/CD147 蛋白, Cynomolgus (HEK293, His)

Basigin, also called CD147 or EMMPRIN, is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily with homology to both the immunoglobulin V domain and major histocompatibility complex class II antigen 尾-chain. Basigin is the receptor for cyclophilins, S100A9 and platelet glycoprotein VI. Basigin tightly associates with monocarboxylate transporters and is essential for their cell surface translocation and activities ^[1][2][3]. Basigin/CD147 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Basigin/CD147 protein, expressed by HEK293 , with C-His labeled tag.

Basigin 具有异构体；普通形式 (basigin 或 basigin-2) 有两个免疫球蛋白结构域，扩展形式 (basigin-1) 有三个。Basigin-1 是视杆细胞衍生视锥细胞活力因子的受体。在同一膜平面中，basigin 还与其他蛋白质结合，包括 GLUT1、CD44 和 CD98。Basigin 的碳水化合物部分被凝集素 (例如 galectin-3 和 E-选择素) 识别。这些分子识别构成了basigin在营养物质运输、炎性白细胞迁移和基质金属蛋白酶诱导中的作用的基础。Basigin 在视觉、精子发生和其他生理现象中很重要，并且在包括癌症在内的许多疾病的发病机制中发挥着重要作用。Basigin 也是一种侵入性蛋白 RH5 的受体，该蛋白存在于疟疾寄生虫中^[1]。
CD147 基因 (称为 BSG) 位于染色体 19p13.3 上，编码 29 kD 蛋白质，但 SDS-PAGE 上的迁移通常发生在 35-65 kD 之间，具体取决于糖基化程度。CD147 Ig 样结构域内已鉴定出三个糖基化位点：两个位于膜近端 Ig 结构域内，一个位于远端 Ig 结构域内^[3]。

Measured by its binding ability in a functional ELISA. When Recombinant SARS-COV-2 S Protein is immobilized at 1 µg/mL (100 µL/well) can bind Recombinant Cynomolgus CD147. The ED₅₀ for this effect is 10.35 μg/mL.

• 
  Measured by its binding ability in a functional ELISA. When Recombinant SARS-COV-2 S Protein is immobilized at 1 µg/mL (100 µL/well) can bind Recombinant Cynomolgus CD147. The ED₅₀ for this effect is 10.35 μg/mL.

Cynomolgus

HEK293

C-6*His

XP_005587354 (A19-H209)

102121721  [NCBI]

AYGAAGTVSTSVENIGSKTLLTCSLNDSSTEVTGHRWLKGGAVLKEDTLPGQKTDFEVDSDDLGGEYSCVFLPEPTGRADIQLDALLSGAPRVKAVKSSEHVSEGETAVLACKSESLPPVTTWVWYKITDSGDQVIVNGSQGRFFVSSSQGRSELRIENLNMEADPGKYACNGTSSEGTDQATVTLRVRSH

Approximately 27-33 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.