BDNF 蛋白, Human (CHO)

BDNF Protein, Human (CHO) is a neurotrophin binding to the high-affinity tropomyosin-related receptor kinase B (TrkB) receptor to regulate neurodevelopmental processes, including neuronal survival, neuronal differentiation, and synaptic plasticity.

重组人 BDNF/脑源性神经营养因子是一种神经营养蛋白，与高亲和力原肌球蛋白相关受体激酶 B (TrkB) 受体结合，调节神经发育过程，包括神经元存活、神经元分化和突触可塑性^{[1]< /sup>。脑源性神经营养因子（BDNF）是哺乳动物中枢神经系统中最丰富的神经营养因子之一，可促进神经元存活和分化。BDNF 在心血管系统中发挥着至关重要的作用[2]}。

The ED₅₀ is <4 μg/ml as measured by C6 cells. Immobilized Human BDNF at 5 μg/ml (100 μl/well) can bind NGF R, Human-Biotin with a linear range of 0.06-4 μg/ml.

Human

CHO

Tag Free

P23560 (H129-R247)

627  [NCBI]

HSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR

12-14 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Li M, et al. Recombinant human brain-derived neurotrophic factor prevents neuronal apoptosis in a novel in vitro model of subarachnoid hemorrhage. Neuropsychiatr Dis Treat. 2017 Apr 3;13:1013-1021.  [Content Brief]

  [2]. Hang P, et al. Brain-derived neurotrophic factor attenuates doxorubicin-induced cardiac dysfunction through activating Akt signalling in rats. J Cell Mol Med. 2017 Apr;21(4):685-696.  [Content Brief]