BDNF 蛋白, Mouse (R129A, R130A, HEK293, His, Solution)

Brain Derived Neurotrophic Factor (BDNF) is a neurotrophin that belongs to NGF-beta family. BDNF can bind to its high affinity receptor TrkB and activates signal transduction cascades (IRS1/2, PI3K, Akt)^[1]. BDNF can also bind to the p75NTR, but the affinity for the p75NTR receptor is lower than for TrkB^[2]. BDNF is a neurotransmitter modulator, and is vital in maturation, survival and differentiation of neuronal populations during development. BDNF also participates in neuronal plasticity, which is essential for learning and memory. BDNF is widely expressed in the CNS^[1]. BDNF Protein, Mouse (R129A, R130A, HEK293, His) is a recombinant mouse BDNF (M1-R249, R129A, R130A) with C-terminal His tag, which is produced in HEK293.

BDNF，一种神经营养因子，属于 NGF-β 家族。BDNF 广泛表达于中枢神经系统、肠道和其他组织中。BDNF 调节神经发育过程，包括神经元群体的成熟、存活和分化以及突触可塑性^[1]。
BDNF 可以与其高亲和力受体 TrkB 结合并激活信号转导级联（IRS1/ 2、PI3K、Akt），从而诱导 Ca²⁺ 摄入增加和转录因子磷酸化。BDNF也可以与p75NTR结合，但对p75NTR受体的亲和力低于对TrkB的亲和力。p75NTR 的激活分别通过激活 c-Jun N 末端激酶 (JNK) 和 NF-κB 表达来增加神经元和神经胶质细胞中的凋亡和炎症信号传导^[2]。在 BDNF 敲除小鼠中，重组 BDNF 可以挽救基础突触传递和海马 LTP 的缺陷^[3]。小鼠 BDNF 与人类具有 >97% 的 aa 序列同一性。小鼠 BDNF 与大鼠具有 >99% 的氨基酸序列同一性。
BDNF 是一种神经递质调节剂，对于发育过程中神经元群的成熟、存活和分化至关重要。BDNF 还参与神经元可塑性，这对于学习和记忆至关重要^[1]。

BDNF (小鼠, 1 ng/mL, 来自 BDNF 敲除小鼠的海马切片, 3 分钟至 2 小时) 显着改善 Schaffer 侧支-CA1 突触的基础突触传递缺陷 ^[3]。

BDNF (小鼠，0.25 μg/侧，海马内给药) 模拟 NSC 移植对 AD 小鼠的有益作用 (改善认知) ^[4]。

Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with TrkB. The ED₅₀ for this effect is 4.391 ng/mL, corresponding to a specific activity is 2.277×10⁵ units/mg.

Mouse

HEK293

C-His

P21237-1 (A19-R249, R129A, R130A)

12064  [NCBI]

Approximately 27.4 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Bathina S, et al. Brain-derived neurotrophic factor and its clinical implications. Arch Med Sci. 2015 Dec 10;11(6):1164-78.  [Content Brief]

  [2]. Lima Giacobbo B, et al. Brain-Derived Neurotrophic Factor in Brain Disorders: Focus on Neuroinflammation. Mol Neurobiol. 2019 May;56(5):3295-3312.  [Content Brief]

  [3]. Patterson SL, et al. Recombinant BDNF rescues deficits in basal synaptic transmission and hippocampal LTP in BDNF knockout mice. Neuron. 1996 Jun;16(6):1137-45.  [Content Brief]

  [4]. Blurton-Jones M, et al. Neural stem cells improve cognition via BDNF in a transgenic model of Alzheimer disease. Proc Natl Acad Sci U S A. 2009 Aug 11;106(32):13594-9.  [Content Brief]