BGLAP 蛋白, Mouse (His-SUMO)

The BGLAP protein, in its carboxylated state, forms an important part of the bone matrix and acts as a negative regulator of bone formation. It limits bone formation and preserves processes such as resorption and mineralization. BGLAP Protein, Mouse (His-SUMO) is the recombinant mouse-derived BGLAP protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of BGLAP Protein, Mouse (His-SUMO) is 46 a.a., with molecular weight of ~24 kDa.

羧化形式的 BGLAP 蛋白是骨基质中的主要有机成分，占总骨蛋白的 1-2%。在这种羧化状态下，它作为骨形成的负调节剂，对于限制骨形成同时保持骨吸收和矿化过程至关重要。值得注意的是，羧化形式对磷灰石和钙表现出很强的结合亲和力。相反，未羧化的形式充当成骨细胞分泌的激素，对多种细胞过程发挥调节影响。这包括它在能量代谢中的作用，它作为一种激素促进胰腺β细胞增殖、胰岛素分泌、胰岛素敏感性和能量消耗。此外，未羧化的骨钙素激素通过与 G 蛋白偶联受体 GPRC6A 相互作用促进睾丸中睾酮的产生，从而在男性生育能力中发挥关键作用。此外，它还充当大脑发育的调节剂，穿过血脑屏障启动信号反应，防止海马神经元凋亡，刺激单胺神经递质的合成，并抑制γ-氨基丁酸（GABA）的合成。重要的是，母体骨钙素在怀孕期间穿过胎盘，在胎儿大脑发育中起着至关重要的作用。

Mouse

E. coli

N-6*His;N-SUMO

P86546 (Y50-I95)

12096  [NCBI]

YLGASVPSPDPLEPTREQCELNPACDELSDQYGLKTAYKRIYGITI

Approximately 24 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm solution of 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.