BID 蛋白, Mouse (His-GST)

BID Protein, a pro-apoptotic member of the Bcl-2 family, is initially discovered through binding to both pro-apoptotic Bax and anti-apoptotic Bcl-2. BID is activated in the BCL-2-regulated or mitochondrial apoptosis pathway and acts as a switch between the extrinsic and intrinsic cell death pathways. BID is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. BID Protein, Mouse (His-GST) is the recombinant mouse-derived BID protein, expressed by E. coli , with N-His, N-GST labeled tag. The total length of BID Protein, Mouse (His-GST) is 195 a.a., with molecular weight of ~48 kDa.

BH3 相互作用结构域死亡激动剂 (BID) 是 Bcl-2 家族的促凋亡成员，最初是通过与促凋亡 Bax 和抗凋亡 Bcl-2 结合而发现的。BID 在 BCL-2 调节或线粒体凋亡途径中被激活，并充当外在和内在细胞死亡途径之间的开关。在细胞凋亡过程中，BID 不仅可以在死亡受体凋亡信号传导过程中被 caspase-8 裂解，还可以被其他 caspases、颗粒酶 B、钙蛋白酶和组织蛋白酶裂解。蛋白酶切割的 BID 迁移到线粒体，在线粒体中诱导依赖于促凋亡蛋白 Bax 和/或 Bak 的线粒体外膜的透化，因此 BID 充当蛋白酶介导的死亡信号的哨兵^[1][2]。

Measured by its binding ability in a functional ELISA. Immobilized mouse BID at 10 μg/mL (100 μL/well) can bind biotinylated human BCL2L1. The ED₅₀ for this effect is 40.75 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized mouse BID at 10μg/mL (100μl/well) can bind biotinylated human BCL2L1,The ED₅₀ for this effect is 40.75 ng/mL.

Mouse

E. coli

N-His;N-GST

EDK99650.1 (M12-D206)

12122  [NCBI]

Approximately 48 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.