BID 蛋白, Mouse (His)

BID Protein, a pro-apoptotic member of the Bcl-2 family, is initially discovered through binding to both pro-apoptotic Bax and anti-apoptotic Bcl-2. BID is activated in the BCL-2-regulated or mitochondrial apoptosis pathway and acts as a switch between the extrinsic and intrinsic cell death pathways. BID is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. BID Protein, Mouse (His) is the recombinant mouse-derived BID protein, expressed by E. coli , with C-His labeled tag. The total length of BID Protein, Mouse (His) is 195 a.a., with molecular weight of ~22.72 kDa.

BH3 相互作用结构域死亡激动剂 (BID) 是 Bcl-2 家族的促凋亡成员，最初是通过与促凋亡 Bax 和抗凋亡 Bcl-2 结合而发现的。BID 在 BCL-2 调节或线粒体凋亡途径中被激活，并充当外在和内在细胞死亡途径之间的开关。在细胞凋亡过程中，BID 不仅可以在死亡受体凋亡信号传导过程中被 caspase-8 裂解，还可以被其他 caspases、颗粒酶 B、钙蛋白酶和组织蛋白酶裂解。蛋白酶切割的 BID 迁移到线粒体，在线粒体中诱导依赖于促凋亡蛋白 Bax 和/或 Bak 的线粒体外膜的透化，因此 BID 充当蛋白酶介导的死亡信号的哨兵^[1][2]。

Mouse BID, His Tag immobilized on CM5 Chip can bind Human BCL2L1/Bcl-XL Protein, His Tag with an affinity constant of 33.06 nM as determined in SPR assay (Biacore T200).

Mouse

E. coli

C-His

EDK99650.1 (A1-S195)

12122

AGSAWSYTACAMDSEVSNGSGLGAKHITDLLVFGFLQSSGCTRQELEVLGRELPVQAYWEADLEDELQTDGSQASRSFNQGRIEPDSESQEEIIHNIARHLAQIGDEMDHNIQPTLVRQLAAQFMNGSLSEEDKRNCLAKALDEVKTAFPRDMENDKAMLIMTMLLAKKVASHAPSLLRDVFHTTVNFINQNLFS

Approximately 22.72 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.