BIN1 蛋白, Human (His)

The BIN1 protein is essential for controlling plasma membrane curvature and shape and is essential for the formation of T-tubules in muscle cells. It acts as a negative regulator of endocytosis and affects intracellular vesicle sorting. BIN1 Protein, Human (His) is the recombinant human-derived BIN1 protein, expressed by E. coli , with N-His labeled tag. The total length of BIN1 Protein, Human (His) is 424 a.a., with molecular weight of ~54 kDa.

BIN1 蛋白在质膜曲率、形状和重塑的调节中发挥着至关重要的作用。在肌肉细胞中，它对于 T 管的形成是不可或缺的，T 管是质膜的管状内陷，对于去极化-收缩耦合至关重要。作为内吞作用的负调节因子，BIN1 还参与细胞内囊泡分选并调节 BACE1 运输，从而影响 β 淀粉样蛋白的产生。在神经元回路中，其在内吞调节中的作用可能会影响 PHF-tau 聚集体的内化。此外，BIN1 参与 MYC 活性的调节，影响细胞增殖，并表现出肌动蛋白成束活性，稳定肌动蛋白丝以防止解聚。BIN1 与 AMPH 形成异二聚体，与 SH3GLB1 结合，与 DNM1、SYNJ1、DNM2、AP2A2、AP2B1、MYC、BIN2、SNX4 和 BACE1 相互作用，表明其参与多种细胞过程和蛋白质-蛋白质相互作用。

Human

E. coli

N-10*His

O00499-10 (M1-P424)

274  [NCBI]

MAEMGSKGVTAGKIASNVQKKLTRAQEKVLQKLGKADETKDEQFEQCVQNFNKQLTEGTRLQKDLRTYLASVKAMHEASKKLNECLQEVYEPDWPGRDEANKIAENNDLLWMDYHQKLVDQALLTMDTYLGQFPDIKSRIAKRGRKLVDYDSARHHYESLQTAKKKDEAKIAKAEEELIKAQKVFEEMNVDLQEELPSLWNSRVGFYVNTFQSIAGLEENFHKEMSKLNQNLNDVLVGLEKQHGSNTFTVKAQPRKKSKLFSRLRRKKNSDNAPAKGNKSPSPPDGSPAATPEIRVNHEPEPAGGATPGATLPKSPSQSSLPAVVVETFPATVNGTVEGGSGAGRLDLPPGFMFKVQAQHDYTATDTDELQLKAGDVVLVIPFQNPEEQDEGWLMGVKESDWNQHKELEKCRGVFPENFTERVP

Approximately 54 kDa

Greater than 85% as determined by reducing SDS-PAGE.

yophilized 粉末.

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCL, 200 mM NaCl, pH 8.0, 10% trehalose, 0.02% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.