BMP-2 蛋白, Human/Mouse/Rat (Tag Free)

Bone morphogenetic protein 2 (BMP-2) is a pleiotropic ligand protein belonging to TNFβ family, and is involved in key embryonic development of vascular and valvular homeostasis. BMP-2 binds to type I receptors (ALK-2/-3/-6) and type II receptors (BMPR2, ACVR2A) to regulate various types of calcification, including atherosclerosis, chronic kidney disease, diabetes, and valve calcification^[1]. BMP-2 is overexpressed by myofibroblast and preosteoblast in the calcified area of human calcified valve, which are densely infiltrated by B lymphocytes and T lymphocytes^[2]. BMP-2 is the junction between atherosclerotic vascular calcification and normal bone formation mechanism^[3]. BMP-2 Protein, Human/Mouse/Rat is 104 a.a. (Q283-R396), expressed in E. coli.

骨形态发生蛋白2（BMP-2）是一种多效性配体蛋白，属于TNFβ家族。BMP-2 格式化 BMP/TGFβ 信号传导以参与血管和瓣膜稳态，这是胚胎发育的关键过程^[1]。
BMP-2/TGFβ 信号传导可通过抑制性终止SMAD 包括 SMAD6 和 SMAD7，它们由 BMP 信号传导激活和诱导，并通过多种机制关闭 BMP 信号传导^[4]。
BMP-2 广泛存在于不同动物中，而其序列人体中的情况与大鼠 (91.86%) 和小鼠 (92.13%) 相似。
BMP 对动脉粥样硬化、肺血管疾病以及血管和瓣膜钙化的病理生理学具有重要贡献^[1]。
BMP-2结合不同的受体，如I型受体（ALK-2/-3/-6）和II型受体（BMPR2、ACVR2A），调节多种钙化类型，包括动脉粥样硬化、慢性肾脏病、糖尿病、瓣膜钙化^[1]。
BMP-2 促进单核细胞浸润和动脉粥样硬化军团的炎症^[5]。
它与通过促炎和促动脉粥样硬化作用增加斑块形成，促进氧化应激、内皮功能障碍和成骨分化^[6]。
BMP-2 在人钙化瓣膜的骨化区域过表达B 淋巴细胞和 T 淋巴细胞密集浸润区域的肌成纤维细胞和前成骨细胞^[2]。
它是动脉粥样硬化血管钙化与正常骨形成机制之间的纽带 ^[3]。
BMP-2 诱导血管生成、内皮细胞 (EC) 增殖和迁移^[7]。
BMP-2 还能增强血管生成、内皮细胞 (EC) 增殖和迁移^[7]。成骨细胞和软骨细胞主转录调节因子 RUNX2 的作用，以依赖于氧化应激和内质网 (ER) 应激的方式促进培养的人冠状血管 SMC 的矿化^[8]。

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is ≤0.5 μg/mL, corresponding to a specific activity is ≥2×10³ U/mg.

• 
  Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.2579 μg/mL, corresponding to a specific activity is 3.877×10³ U/mg.

Rat; Mouse; Human

E. coli

Tag Free

P12643 (Q283-R396)

650

QAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR

Approximately 12-13 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from sterile 50 mM Tris-HCL, 200 mM NaCL, 500 mM arginine, pH 8.0 or 50 mM Tris-HCL, 200 mM NaCl, 500 mM arginine, pH 8.0, 5% trehalose or 0.1% TFA, 30% Acetonitrile, pH 2.5 or pH 2.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.