BMP-3B/GDF10 蛋白, Human (His)

Bone morphogenetic protein 3 (BMP-3; GDF10) is a polymorphic ligand protein belonging to the TGF-β family. BMP-3 is the main component of osteoblast and has osteogenic activity^[1]. BMP-3 plays an inhibitory role in the carcinogenic process, and inhibits the occurrence of colon tumors through ActRIIB/ SMad2-dependent and TAK1/JNK signaling pathways^[2]. BMP-3B/GDF10 Protein, Human has a total length of 110 amino acids (Q369-R478), is expressed in E. coli cells.

骨形态发生蛋白3（BMP-3）是一种多效性配体蛋白，属于TGFβ家族。BMP-3是成骨素的主要成分，具有成骨活性^[1]。BMP-3广泛存在于不同的动物中，而人类的序列与大鼠（81.94%）和小鼠（80.86%）的相似度较低。
BMP-3尤其可作为筛查结直肠癌的可靠生物标志物（ CRC），因为 BMP-3 在癌细胞系中高度甲基化，其蛋白表达显着降低^[2]。
BMP-3 在癌变中也发挥着抑制作用，通过 ActRIIB 抑制结肠肿瘤的发生/SMAD2 依赖性和 TAK1/JNK 信号通路^[2]。
BMP-3 可对不同细胞类型中的激活素信号发挥双向调节作用。BMP-3 刺激人间充质干细胞的增殖，这种增殖可被 TGF-β/激活素受体激酶抑制剂阻断^[3]。
BMP/TGFβ 信号传导参与血管和瓣膜稳态，这是胚胎发育的关键过程^[4]。BMP/TGFβ 信号传导可以被包括 SMAD6 和 SMAD7 在内的抑制性 SMAD 终止，这些 SMAD 信号被 BMP 信号传导激活和诱导，并通过多种机制关闭 BMP 信号传导^[5]。

BMP-3 (1, 10, 50, and 10 ng/mL; 7, 14, and 21 d) alone and in combination with 10 ng/mL TGF-β promotes differentiation of human mesenchymal stem cells to a nucleus poposus phenotype^[6].
BMP-3 (1, 2.5, and 10 ng/mL; 3 d) inhibits human bone marrow stromal cell (HBMSC) growth in a dose-dependent manner^[7].

Human

E. coli

N-10*His

P55107 (Q369-R478)

2662  [NCBI]

MQWDEPRVCSRRYLKVDFADIGWNEWIISPKSFDAYYCAGACEFPMPKIVRPSNHATIQSIVRAVGIIPGIPEPCCVPDKMNSLGVLFLDENRNVVLKVYPNMSVDTCACR

Approximately 16 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Yang P, et al. The role of bone morphogenetic protein signaling in vascular calcification. Bone. 2020 Dec;141:115542.  [Content Brief]

  [2]. Miyazawa K, et al. Regulation of TGF-β Family Signaling by Inhibitory Smads. Cold Spring Harb Perspect Biol. 2017 Mar 1;9(3):a022095.  [Content Brief]

  [3]. Wen J, et al. BMP3 suppresses colon tumorigenesis via ActRIIB/SMAD2-dependent and TAK1/JNK signaling pathways. J Exp Clin Cancer Res. 2019 Oct 28;38(1):428.  [Content Brief]

  [4]. Bahamonde ME, et al. BMP3: to be or not to be a BMP. J Bone Joint Surg Am. 2001;83-A Suppl 1(Pt 1):S56-62.  [Content Brief]

  [5]. Stewart A, et al. BMP-3 promotes mesenchymal stem cell proliferation through the TGF-beta/activin signaling pathway. J Cell Physiol. 2010 Jun;223(3):658-66.  [Content Brief]

  [6]. Zhou X, et al. BMP3 Alone and Together with TGF-β Promote the Differentiation of Human Mesenchymal Stem Cells into a Nucleus Pulposus-Like Phenotype. Int J Mol Sci. 2015 Aug 27;16(9):20344-59.  [Content Brief]

  [7]. Faucheux C, et al. Opposing actions of BMP3 and TGFβ1 in human bone marrow stromal cell growth and differentiation[J]. Biochemical and biophysical research communications, 1997, 241(3): 787-793.